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      Acute and Chronic Effects of Hyperosmolality on mRNA and Protein Expression and the Activity of Na-K-ATPase in the IMCD

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          Abstract

          We investigated acute and chronic effects of hyperosmolality on mRNA and protein expressions of Na-K-ATPase α and β isoforms and Na-K-ATPase activity in the rat inner medullary collecting duct (IMCD). Incubation of IMCD in hypertonic medium for 30 min reduced the Na-K-ATPase activity by 50%. The Na-K-ATPase activity of dehydrated rats measured in isotonic medium was decreased, and incubation in hypertonic medium did not further decrease the activity. Incubation of IMCD in hypertonic medium for 6 h did not change α<sub>1</sub> mRNA. In contrast, dehydration decreased α<sub>1</sub> subunit mRNA and protein and β<sub>1</sub> protein expressions without changing β<sub>1</sub> mRNA. These data show (1) that acute hyperosmolality decreases Na-K-ATPase activity in IMCD without changing α<sub>1</sub> and β<sub>1</sub> mRNA and (2) that 2 days of dehydration decreased Na-K-ATPase activity by reducing α<sub>1</sub> and β<sub>1</sub> proteins. Thus, the mechanisms for the inhibition of the Na-K-ATPase activity in IMCD is defferent between acute and chronic exposure to hyperosmolality.

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          Author and article information

          Journal
          EXN
          Nephron Exp Nephrol
          10.1159/issn.1660-2129
          Cardiorenal Medicine
          S. Karger AG
          1660-2129
          1999
          August 1999
          26 July 1999
          : 7
          : 4
          : 295-305
          Affiliations
          a2nd Department of Internal Medicine,Tokyo Medical and Dental University, Tokyo; b3rd Department of Internal Medicine, Kumamoto University School of Medicine, Kumamoto, Japan
          Article
          20617 Exp Nephrol 1999;7:295–305
          10.1159/000020617
          10450017
          e1f8caaf-cdb4-4d76-ab17-ae0ac5d9885e
          © 1999 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Page count
          Figures: 12, References: 31, Pages: 11
          Categories
          Original Paper

          Cardiovascular Medicine,Nephrology
          Inner medullary collecting duct,Hyperosmolality,α isoform,Na-K-ATPase,β isoform

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