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      Virus-induced gene silencing using begomovirus satellite molecules.

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          Abstract

          Virus-induced gene silencing (VIGS) has emerged as a powerful method for studying gene function. VIGS is induced by infecting a plant with a plant virus that has had its genome modified to include a sequence from the host gene to be silenced. DNAβ and DNA1 are satellite and single-stranded DNA molecules associated with begomoviruses (family Geminiviridae). We converted DNAβ and DNA1 into gene-silencing vectors. The VIGS vectors can induce silencing efficiently in many solanaceous plants. Here, we describe procedures for the use of these two gene-silencing vectors for VIGS in different hosts.

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          Author and article information

          Journal
          Methods Mol Biol
          Methods in molecular biology (Clifton, N.J.)
          Springer Science and Business Media LLC
          1940-6029
          1064-3745
          2012
          : 894
          Affiliations
          [1 ] Institute of Biotechnology, Zhejiang University, Hangzhou, China. zzhou@zju.edu.cn
          Article
          10.1007/978-1-61779-882-5_4
          22678572
          e20e07c7-45b5-47a4-b4ed-a5bad7cce3c8
          History

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