Trunk electromyographic signals (EMG) are often contaminated with heart muscle electrical activity (ECG) due to the proximity of the collection sites to the heart and the volume conduction characteristics of the ECG through the torso. Few studies have quantified ECG removal techniques relative to an uncontaminated EMG signal (gold standard or criterion measure), or made direct comparisons between different methods for a given set of data. Understanding the impacts of both untreated contaminated EMG and ECG elimination techniques on the amplitude and frequency parameters is vital given the widespread use of EMG. The purpose of this study was to evaluate four groups of current and commonly used techniques for the removal of ECG contamination from EMG signals. ECG recordings at two intensity levels (rest and 50% maximum predicted heart rate) were superimposed on 11 uncontaminated biceps brachii EMG signals (rest, 7 isometric and 3 isoinertial levels). The 23 removal methods used were high pass digital filtering (finite impulse response (FIR) using a Hamming window, and fourth-order Butterworth (BW) filter) at five cutoff frequencies (20, 30, 40, 50, and 60 Hz), template techniques (template subtraction and an amplitude gating template), combinations of the subtraction template and high pass digital filtering, and a frequency subtraction/signal reconstruction method. For muscle activation levels between 10% and 25% of maximum voluntary contraction, the template subtraction and BW filter with a 30 Hz cutoff were the two best methods for maximal ECG removal with minimal EMG distortion. The BW filter with a 30 Hz cutoff provided the optimal balance between ease of implementation, time investment, and performance across all contractions and heart rate levels for the EMG levels evaluated in this study.