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      Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets.

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          Abstract

          ATG2 is one of the autophagy-related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N-terminal amino acids 1-198 and the C-terminal amino acids 1830-1938 are required for the localization to isolation membranes and lipid droplets, respectively. The C-terminal region is not required for the localization to isolation membranes and for autophagy. We also identified an amphipathic helix in ATG2A that is required for both its localization to organelles and autophagosome formation. These data suggest that the dual localization of ATG2A is regulated by different regions.

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          Author and article information

          Journal
          FEBS Lett.
          FEBS letters
          Wiley
          1873-3468
          0014-5793
          December 2017
          : 591
          : 23
          Affiliations
          [1 ] Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Japan.
          [2 ] Department of Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
          [3 ] Microscopy Research Support Unit Research Core, Tokyo Medical and Dental University, Japan.
          Article
          10.1002/1873-3468.12901
          29113029
          e2468bbc-929a-44aa-88ee-ef8562170757
          History

          amphipathic helix,VPS13,ATG2,lipid droplet,autophagy
          amphipathic helix, VPS13, ATG2, lipid droplet, autophagy

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