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      Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium

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          Abstract

          Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth, respectively. The polysaccharides showed degree of deacetilation and viscosimetric molecular weight as: 6.2% and 3.25 x10(4) g/mol for chitin, and 85% and 2.72 x 10(4) g/mol for chitosan, respectively. The results obtained suggest high biotechnological potential of yam bean as an economic source to produce chitin and chitosan by C. elegans. In addition, the new medium using yam bean for production of the chitin and chitosan may be used for many purposes to reduce the cost price of fermentation processes.

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          Chitin Chemistry

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            ATIVIDADE ENZIMÁTICA DE MICRORGANISMOS ISOLADOS DO JACATUPÉ (Pachyrhizus erosus L. Urban)

            O isolamento e a identificação de microrganismos produtores de enzimas de interesse comercial, utilizando tubérculos de jacatupé (Pachyrhizus erosus L. Urban), foi o objetivo principal deste trabalho. Isolaram-se microrganismos endofíticos e epifíticos identificados por observação micromorfológica. A avaliação da atividade enzimática das linhagens foi determinada pelo método de difusão em ágar. As sessenta e oito linhagens isoladas dos tubérculos de jacatupé foram cultivadas em meio sólido específico para amilase, lipase, protease e celulase por 96h a 280 C. Os microrganismos epifíticos encontrados foram Pithomyces (7,3%), Aspergillus (19,2%), Fusarium (5,9%) e Trichoderma (5,8%), e os endofíticos foram Mucor (7,3%), Rhizopus (10,3%), Bacillus (19,0%), Staphylococcus (10,3%) e Nocardiopsis (15%). As linhagens de Nocardiopsis sp. apresentaram atividade lipolítica superior à do padrão, porém a atividade amilolítica não apresentou diferença significativa comparada com o padrão. As linhagens de Mucor sp., Pithomyces sp. e Staphylococcus sp. produziram atividade proteolítica abaixo do padrão. Nenhum isolado apresentou atividade celulolítica.
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              Isolation of Chitin and Chitosan from Honeybees

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                ejb
                Electronic Journal of Biotechnology
                Electron. J. Biotechnol.
                Pontificia Universidad Católica de Valparaíso and CONICYT (Valparaíso )
                0717-3458
                January 2007
                : 10
                : 1
                : 61-68
                Affiliations
                [1 ] Universidade Católica de Pernambuco Brazil
                [2 ] Universidade Federal de Pernambuco Brazil
                [3 ] Universidade Federal Rural de Pernambuco Brazil
                [4 ] Universidade Federal de Pernambuco Brazil
                [5 ] Universidade Católica de Pernambuco Brazil
                Article
                S0717-34582007000100006
                10.4067/S0717-34582007000100006
                Product
                Product Information: website
                Categories
                BIOTECHNOLOGY & APPLIED MICROBIOLOGY

                Biotechnology

                biopolymers, chitin, chitosan, Cunninghamella elegans, Zygomycetes

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