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      ACTIVIDAD DE LA LACTICO DEHIDROGENASA (LDH) EN EMERITA ANALOGA (STIMPSON 1857) CRUSTACEA, HIPPIDAE Translated title: LACTIC DEHYDROGENASE ACTIVITY (LDH) IN EMERITA ANALOGA (STIMPSON 1857) CRUSTACEA, HIPPIDAE

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          Abstract

          Emerita analoga (Stimpson 1857) habita en la zona infralitoral de playas arenosas, desarrollando intensa movilidad en la zona de barrido y embate de las olas. Su habilidad motriz, reflejada en una constante actividad muscular explosiva, sugiere una alta actividad glicolítica (fermentativa) complementaria a la fosforilación oxidativa. En este trabajo se determinó la actividad específica aparente de la láctico dehidrogenasa (LDH EC 1.1.1.27) de E. analoga como indicador de hipoxia funcional. Además, se estudió el efecto del peso y del pH sobre la actividad LDH, así como en los valores aparentes de sus parámetros cinéticos (Vmax y Km). Los resultados indican que los organismos grandes ( a 1,5) y pequeños (< a 1,5 mm) de E. analoga, se ajustan a una curva alométrica actividad - peso positiva (p = 0,0108). El pH óptimo de la LDH es 7,8 para animales pequeños y de 5,5 en animales grandes. Esto sugiere que la acidosis metabólica podría ser regulada a escala enzimática en los animales pequeños y como tampón muscular en los grandes. Para los dos tamaños la LDH mostró una cinética Michaeliana, siendo el Vmax de los animales grandes de un orden de magnitud mayor que en los pequeños (1,043 y 0,121 mmol min-1 g wet wt-1 respectivamente). Sin embargo, se observó una mayor afinidad (menor valor de la constante de Michaelis) en los organismos pequeños que en los grandes (0,076 y 0,094 mM respectivamente). Finalmente, la pendiente positiva de la curva actividad - peso concuerda con la alometría del metabolismo anaeróbico escasamente descrito e inverso a la pendiente del aeróbico. Esto sugiere que la LDH tendría un rol importante en la movilidad de E. analoga durante su ontogenia en la zona infralitoral

          Translated abstract

          Emerita analoga (Stimpson 1857) inhabitant sandy beaches, it develops intense activity in swatch and fetch embattle zone of the waves. Its motility reflected by a constant and burst muscular activity, suggests a high glycolitic (fermentative) activity, complementary to the oxidative phosphorilation. In this study, the apparent specific activity of the enzyme lactic dehydrogenase (LDH, EC 1.1.1.27) of E. Analoga was tested, as indicator of functional hypoxia. In addition, the effect of weight and pH on LDH activity was studied, and also the apparent values of its kinetic parameters (Vmax y Km). Our results indicated that apparent specific LDH activity in bigger (1,5 mm) and smaller (<1,5 mm in length) organisms of E. analoga, correspond to a positive allometric pattern (p = 0,0108). The optimal pH for the apparent LDH activity best activity was 7,8 in smaller animals and 5,5 in bigger ones. The results suggest that metabolic acidosis could be regulated at enzymatic level in small animals, and as a muscular buffer in the bigger ones. For the sizes ranges used, enzyme activity showed a Michaelian-Menten kinetic. Vmax value in larger animals was one order of magnitude higher than smaller ones (1,043 and 0,121 mmol min-1 g wet wt-1, respectively). However, we observed a high affinity constant in small organisms than in bigger ones (0,076 and 0,094 mM, respectively). Finally, the positive slope of the curve activity /weight is consistent with the positive allometry described for the anaerobic metabolism, scarcely present in literature. Our results suggest an important role of the LDH activity on the motility of E. analoga during its ontogenetic development in the infra-littoral zone

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          Most cited references 23

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                gayana
                Gayana (Concepción)
                Gayana (Concepc.)
                Universidad de Concepción.<br>Facultad de Ciencias Naturales y Oceanográficas. (Concepción )
                0717-6538
                2003
                : 67
                : 1
                : 68-74
                Affiliations
                [1 ] Universidad de Concepción Chile
                Article
                S0717-65382003000100009
                10.4067/S0717-65382003000100009
                Product
                Product Information: website
                Categories
                ZOOLOGY

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