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      Blood-Collecting Methodology Does Not Affect the Maturational Pattern of Serum Hormone Concentrations (Testosterone, LH, FSH and Prolactin) in the Male Rat

      ,

      Neuroendocrinology

      S. Karger AG

      Blood collection, Testosterone, LH, FSH, Prolactin, Male rat, Ether stress

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          Abstract

          Serum hormone levels of blood collected within 1 min by cardiac puncture following ether anesthesia were compared to those of blood collected by decapitation in male rats 30, 60, and 90 days of age. Neither testosterone (T) nor LH was affected by the method of blood collection. Serum FSH was significantly elevated in blood collected by cardiac puncture, and 30-day old rats were the most sensitive to this effect. Serum prolactin (Prl) was significantly elevated in all age groups when blood was collected by cardiac puncture. Both blood-collecting methods demonstrated that, in the 3 ages tested, serum T increased with age, LH and FSH decreased with age, and Prl did not change with age, indicating that blood collection by cardiac puncture is as valid a method as is decapitation for assessing serum hormone levels during maturation.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1978
          1978
          26 March 2008
          : 26
          : 4
          : 202-207
          Affiliations
          Department of Biological Sciences, State University of New York, Binghamton, N.Y.
          Article
          122827 Neuroendocrinology 1978;26:202–207
          10.1159/000122827
          687389
          © 1978 S. Karger AG, Basel

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          Pages: 6
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