+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Binding of Gold-Protein Ligand Complexes to Neurokinin Receptors

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Active neurokinin (NK) receptors were visualized with 5-nm colloidal gold-protein-substance P (GPSP) and -senktide (GPSenk) complexes on vascular tissues. Electron micrographs of pig coronary strips incubated with GPSP showed gold particles either bound to the plasmalemma or inside intracytoplasmic vesicles of endothelial cells. Preincubation with SP or the NK<sub>1</sub> receptor antagonist L-703606 prevented GPSP marking. No gold particles were seen after incubation with GPSenk. On coronary strips in vitro, which had been precontracted with U46619, GPSP induced relaxations similar to those produced by equimolar concentrations of SP, both relaxations being inhibited by L-703606. Analogous to senktide, GPSenk was totally inactive on arterial strips. Incubation of rat portal veins with GPSenk showed gold particles bound to the plasmalemma or inside intracytoplasmic vesicles of smooth muscle cells. Preincubation with senktide or the NK<sub>3</sub> receptor antagonist R-820 prevented GPSenk marking. On portal veins in vitro GPSenk induced contractions similar to those induced by equimolar concentrations of senktide or NKB; these effects were inhibited by R-820. Our results show that colloidal gold-protein complexes present biological activity and selectivity similar to those of their respective native ligand and detect the presence of active receptors; in addition, they suggest the presence of NK-receptor-mediated endocytosis in endothelial cells of coronary artery and in smooth muscle cells of the portal vein.

          Related collections

          Most cited references 4

          • Record: found
          • Abstract: not found
          • Article: not found

          The rat isolated portal vein: a preparation sensitive to neurokinins, particularly neurokinin B

            • Record: found
            • Abstract: not found
            • Article: not found

            Autoradiographic demonstration of endothelium-dependent 125I-Bolton-Hunter substance P binding to dog carotid artery.

              • Record: found
              • Abstract: not found
              • Article: not found

              Autoradiographic analysis of receptors on vascular endothelium


                Author and article information

                J Vasc Res
                Journal of Vascular Research
                S. Karger AG
                February 1999
                01 March 1999
                : 36
                : 1
                : 59-67
                aDepartment of Psychiatry, Geneva University Hospital, Geneva, bAssociation for International Research on Regeneration, Cologny, cDepartment of Pharmacology, University of Geneva, dDivision of Surgical Investigations, Department of Surgery, Geneva University Hospital, Geneva, Switzerland
                25626 J Vasc Res 1999;36:59–67
                © 1999 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 6, Tables: 1, References: 30, Pages: 9
                Research Paper


                Comment on this article