5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      Collision-Induced Unfolding of Partially Metalated Metallothionein-2A: Tracking Unfolding Reactions of Gas-Phase Ions

      1 , 1 , 1
      Analytical Chemistry
      American Chemical Society (ACS)

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          <p class="first" id="P1">Metallothioneins (MTs) constitute a group of intrinsically disordered proteins that exhibit extreme diversity in structure, biological functionality, and metal ion specificity. Structures of coordinatively saturated metalated MTs have been extensively studied, but very limited structural information for the partially metalated MTs exists. Here, the conformational preferences from partial metalation of rabbit metallothionein-2A (MT) by Cd <sup>2+</sup>, Zn <sup>2+</sup>, and Ag <sup>+</sup> are studied using nanoelectrospray ionization ion mobility mass spectrometry. We also employ collision-induced unfolding to probe differences in the gas-phase stabilities of these partially metalated MTs. Our results show that despite their similar ion mobility profiles, Cd <sub>4</sub>-MT, Zn <sub>4</sub>-MT, Ag <sub>4</sub>-MT, and Ag <sub>6</sub>-MT differ dramatically in their gas-phase stabilities. Furthermore, the sequential addition of each Cd <sup>2+</sup> and Zn <sup>2+</sup> ion results in the incremental stabilization of unique unfolding intermediates. </p><p id="P2"> <div class="figure-container so-text-align-c"> <img alt="" class="figure" src="/document_file/7f11dc09-b6ce-4a40-896a-888ea3990209/PubMedCentral/image/nihms-1020389-f0001.jpg"/> </div> </p>

          Related collections

          Author and article information

          Journal
          Analytical Chemistry
          Anal. Chem.
          American Chemical Society (ACS)
          0003-2700
          1520-6882
          October 2018
          October 2018
          Affiliations
          [1 ]Department of Chemistry, Texas A&amp;M University, College Station, Texas 77843, United States
          Article
          10.1021/acs.analchem.8b01622
          6463490
          30221929
          e37e134b-934c-4b82-9d08-2992cebb866f
          © 2018
          History

          Comments

          Comment on this article