16
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Microbial community dynamics in an ANAMMOX reactor for piggery wastewater treatment with startup, raising nitrogen load, and stable performance

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Bacterial community dynamics of the ANAMMOX reactor of an integrated “UASB + SHARON + ANAMMOX” system for treating piggery wastewater were investigated using the Illumina MiSeq method with samples obtained at ~ 2-week intervals during a 314-day period. With aerobic activated sludge as seeds and low content artificial wastewater (NH 4 +–N 50 mg/L; NO 2 –N 55 mg/L) as influent for the ANAMMOX reactor, nitrogen removal was initially observed on day 38 with a removal rate 1.3 mg N L −1 day −1, and increased to 90.4 mg N L −1 day −1 on day 55 with almost complete removal of ammonia and nitrite, indicating a successful startup of the reactor. Increasing influent load stepwise to NH 4 +–N 272.7 mg/L/NO 2 –N 300 mg/L, nitrogen removal rate increased gradually to 470 mg N L −1 day −1 on day 228, and maintained a stable level (~ 420 mg N L −1 day −1) following introduction of SHARON effluent since day 229. Correlation between microbial community dynamics and nitrogen removal capability was significant ( r = 0.489, p < 0.001). Microbial community composition was determined by influent ammonia, influent nitrite, effluent nitrate and some undefined factors. Anammox bacteria, accounting for ~ 98.7% of Planctomycetes, became detectable (0.03% relative abundance) since day 38 and increased to 0.9% on day 58, well consistent with nitrogen removal performance of the reactor. Relative abundance of anammox bacteria gradually increased to 38.4% on day 140 with stepwise increased influent load; decreased to 0.4% on day 169 because of nitrite inhibition; increased to 19.24% on day 233 when the influent load was dropped; kept at ~ 9.0% with SHARON effluent used as influent and dropped to 3.3% finally. Anammox bacteria, only Candidatus Brocadia and Ca. Kuenenia detected, were the most abundant at genus level. Ca. Brocadia related taxa were enriched firstly under low load and detectable during the entire experimental period. Three main groups represented by Ca. Brocadia related OTUs were enriched or eliminated at different loads, but Ca. Kuenenia related taxa were enriched only under high load (NO 2 –N > 300 mg/L), suggesting their different niches and application for different loads. These findings improve the understanding of relationships among microbial community/functional taxa, running parameters and reactor performance, and will be useful in optimizing running parameters for rapid startup and high, stable efficiency.

          Electronic supplementary material

          The online version of this article (10.1186/s13568-018-0686-0) contains supplementary material, which is available to authorized users.

          Related collections

          Most cited references48

          • Record: found
          • Abstract: not found
          • Article: not found

          QIIME allows analysis of high-throughput community sequencing data.

            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms

              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Metabolic network analysis reveals microbial community interactions in anammox granules

              Microbial communities mediating anaerobic ammonium oxidation (anammox) represent one of the most energy-efficient environmental biotechnologies for nitrogen removal from wastewater. However, little is known about the functional role heterotrophic bacteria play in anammox granules. Here, we use genome-centric metagenomics to recover 17 draft genomes of anammox and heterotrophic bacteria from a laboratory-scale anammox bioreactor. We combine metabolic network reconstruction with metatranscriptomics to examine the gene expression of anammox and heterotrophic bacteria and to identify their potential interactions. We find that Chlorobi-affiliated bacteria may be highly active protein degraders, catabolizing extracellular peptides while recycling nitrate to nitrite. Other heterotrophs may also contribute to scavenging of detritus and peptides produced by anammox bacteria, and potentially use alternative electron donors, such as H2, acetate and formate. Our findings improve the understanding of metabolic activities and interactions between anammox and heterotrophic bacteria and offer the first transcriptional insights on ecosystem function in anammox granules.
                Bookmark

                Author and article information

                Contributors
                15250967477@163.com
                duweili789@163.com
                miaoll@im.ac.cn
                liuying@im.ac.cn
                +86-10-64806081 , liuzhp@im.ac.cn
                Journal
                AMB Express
                AMB Express
                AMB Express
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                2191-0855
                1 October 2018
                1 October 2018
                2018
                : 8
                : 156
                Affiliations
                [1 ]ISNI 0000000119573309, GRID grid.9227.e, State Key Laboratory of Microbial Resources, Institute of Microbiology, , Chinese Academy of Sciences, ; No. 1 West Beichen Road, Chaoyang District, Beijing, 100101 People’s Republic of China
                [2 ]ISNI 0000 0004 1797 8419, GRID grid.410726.6, University of Chinese Academy of Sciences, ; Beijing, 100049 People’s Republic of China
                Article
                686
                10.1186/s13568-018-0686-0
                6167272
                30276499
                e38972dd-14b4-4821-a712-4e337e119ffc
                © The Author(s) 2018

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

                History
                : 20 August 2018
                : 24 September 2018
                Categories
                Original Article
                Custom metadata
                © The Author(s) 2018

                Biotechnology
                anammox,anammox bacteria,microbial community dynamics,stepwise increasing load,piggery wastewater

                Comments

                Comment on this article