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      Isolation and characterization of polysaccharides of a hybrid mushroom (backcross mating between PfloVv12 and Volvariella volvacea).

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          Abstract

          Three polysaccharide fractions (PS-I, PS-II, and PS-III) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-β glucan. PS-II and PS-III were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as;

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          Author and article information

          Journal
          Carbohydr. Res.
          Carbohydrate research
          Elsevier BV
          1873-426X
          0008-6215
          Nov 08 2011
          : 346
          : 15
          Affiliations
          [1 ] Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
          Article
          S0008-6215(11)00414-9
          10.1016/j.carres.2011.08.019
          21924407
          e3f000b2-3184-4e19-89c8-a88f12f90617
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