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      The human NAD metabolome: Functions, metabolism and compartmentalization

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          Abstract

          The metabolism of NAD has emerged as a key regulator of cellular and organismal homeostasis. Being a major component of both bioenergetic and signaling pathways, the molecule is ideally suited to regulate metabolism and major cellular events. In humans, NAD is synthesized from vitamin B3 precursors, most prominently from nicotinamide, which is the degradation product of all NAD-dependent signaling reactions. The scope of NAD-mediated regulatory processes is wide including enzyme regulation, control of gene expression and health span, DNA repair, cell cycle regulation and calcium signaling. In these processes, nicotinamide is cleaved from NAD + and the remaining ADP-ribosyl moiety used to modify proteins (deacetylation by sirtuins or ADP-ribosylation) or to generate calcium-mobilizing agents such as cyclic ADP-ribose. This review will also emphasize the role of the intermediates in the NAD metabolome, their intra- and extra-cellular conversions and potential contributions to subcellular compartmentalization of NAD pools.

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          Most cited references 136

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          Visfatin: a protein secreted by visceral fat that mimics the effects of insulin.

          Fat tissue produces a variety of secreted proteins (adipocytokines) with important roles in metabolism. We isolated a newly identified adipocytokine, visfatin, that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to a protein identified previously as pre-B cell colony-enhancing factor (PBEF), a 52-kilodalton cytokine expressed in lymphocytes. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Mice heterozygous for a targeted mutation in the visfatin gene had modestly higher levels of plasma glucose relative to wild-type littermates. Surprisingly, visfatin binds to and activates the insulin receptor. Further study of visfatin's physiological role may lead to new insights into glucose homeostasis and/or new therapies for metabolic disorders such as diabetes.
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            Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology.

            The membrane proteins CD38 and CD157 belong to an evolutionarily conserved family of enzymes that play crucial roles in human physiology. Expressed in distinct patterns in most tissues, CD38 (and CD157) cleaves NAD(+) and NADP(+), generating cyclic ADP ribose (cADPR), NAADP, and ADPR. These reaction products are essential for the regulation of intracellular Ca(2+), the most ancient and universal cell signaling system. The entire family of enzymes controls complex processes, including egg fertilization, cell activation and proliferation, muscle contraction, hormone secretion, and immune responses. Over the course of evolution, the molecules have developed the ability to interact laterally and frontally with other surface proteins and have acquired receptor-like features. As detailed in this review, the loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications in mice. CD38 is a powerful disease marker for human leukemias and myelomas, is directly involved in the pathogenesis and outcome of human immunodeficiency virus infection and chronic lymphocytic leukemia, and controls insulin release and the development of diabetes. Here, the data concerning diseases are examined in view of potential clinical applications in diagnosis, prognosis, and therapy. The concluding remarks try to frame all of the currently available information within a unified working model that takes into account both the enzymatic and receptorial functions of the molecules.
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              Nutrient-sensitive mitochondrial NAD+ levels dictate cell survival.

              A major cause of cell death caused by genotoxic stress is thought to be due to the depletion of NAD(+) from the nucleus and the cytoplasm. Here we show that NAD(+) levels in mitochondria remain at physiological levels following genotoxic stress and can maintain cell viability even when nuclear and cytoplasmic pools of NAD(+) are depleted. Rodents fasted for 48 hr show increased levels of the NAD(+) biosynthetic enzyme Nampt and a concomitant increase in mitochondrial NAD(+). Increased Nampt provides protection against cell death and requires an intact mitochondrial NAD(+) salvage pathway as well as the mitochondrial NAD(+)-dependent deacetylases SIRT3 and SIRT4. We discuss the relevance of these findings to understanding how nutrition modulates physiology and to the evolution of apoptosis.
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                Author and article information

                Journal
                Crit Rev Biochem Mol Biol
                Crit. Rev. Biochem. Mol. Biol
                IBMG
                ibmg20
                Critical Reviews in Biochemistry and Molecular Biology
                Informa Healthcare
                1040-9238
                1549-7798
                4 July 2015
                2 April 2015
                : 50
                : 4
                : 284-297
                Affiliations
                [ a ]Institute of Nanobiotechnologies, St. Petersburg State Polytechnical University St. PetersburgRussia
                [ b ]Institute of Cytology, Russian Academy of Sciences St. PetersburgRussia
                [ c ]Department of Molecular Biology, University of Bergen BergenNorway
                Author notes
                Address for correspondence: Mathias ZieglerDepartment of Molecular Biology, University of Bergen Thormøhlensgate 55 5008 BergenNorway. Tel: +47 555 84591. Fax: +47 555 89683. E-mail: mathias.ziegler@ 123456mbi.uib.no
                Andrey NikiforovInstitute of Nanobiotechnologies, St. Petersburg State Polytechnical University Polytechnicheskaya 29, 195251 St. PetersburgRussia. Tel: +7 812 5529809. Fax: +7 812 5528579. E-mail: andrey.nikiforov@ 123456gmail.com
                Article
                1028612
                10.3109/10409238.2015.1028612
                4673589
                25837229
                The Author(s). Published by Taylor & Francis.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License ( http://creativecommons.org/Licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

                Page count
                Pages: 14
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