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      Sodium Hyaluronate Eye Drop. A Scanning and Transmission Electron Microscopy Study of the Corneal Surface

      ,

      Ophthalmic Research

      S. Karger AG

      Sodium hyaluronate, Mouse, Cornea, Ocular mucus, Scanning microscopy, Transmission microscopy

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          Abstract

          The association with time (0–60 min) of an eye drop preparation containing sodium hyaluronate with the corneal epithelial cell surface was examined using both cryo-fixation and osmium tetroxide vapor fixation and scanning (SEM) and transmission electron microscopy (TEM). In phosphate-buffered saline (PBS)-treated control eyes of adult mice, 37 postnatal days (PND) old, a variably thick, but well-defined preocular mucus coat was seen, while 5-PND-old animals treated with PBS lacked morphologically detectable mucus at the corneal surface. In mice of both ages, the eye drop preparation was detectable at the corneal surface at time 0 and 5, 15, 30 and 60 min after its application. For these time periods, in both the adult and the pup, the eye drop preparation containing sodium hyaluronate appeared to be composed of electron-dense filamentous material (TEM) which associated with corneal surface cell microvilli. SEM revealed that the eye drop preparation was globular in composition and that its distribution became patchy over the cornea with time for both the adult and the pup.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1987
          1987
          04 December 2009
          : 19
          : 5
          : 277-284
          Affiliations
          Department of Anatomy/Cell Biology, School of Medicine, Wayne State University, Detroit, Mich., USA
          Article
          265507 Ophthalmic Res 1987;19:277–284
          10.1159/000265507
          3438046
          © 1987 S. Karger AG, Basel

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          Page count
          Pages: 8
          Categories
          Original Paper

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