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      Sodium Hyaluronate Eye Drop. A Scanning and Transmission Electron Microscopy Study of the Corneal Surface

      Ophthalmic Research
      S. Karger AG
      Sodium hyaluronate, Mouse, Cornea, Ocular mucus, Scanning microscopy, Transmission microscopy

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          The association with time (0–60 min) of an eye drop preparation containing sodium hyaluronate with the corneal epithelial cell surface was examined using both cryo-fixation and osmium tetroxide vapor fixation and scanning (SEM) and transmission electron microscopy (TEM). In phosphate-buffered saline (PBS)-treated control eyes of adult mice, 37 postnatal days (PND) old, a variably thick, but well-defined preocular mucus coat was seen, while 5-PND-old animals treated with PBS lacked morphologically detectable mucus at the corneal surface. In mice of both ages, the eye drop preparation was detectable at the corneal surface at time 0 and 5, 15, 30 and 60 min after its application. For these time periods, in both the adult and the pup, the eye drop preparation containing sodium hyaluronate appeared to be composed of electron-dense filamentous material (TEM) which associated with corneal surface cell microvilli. SEM revealed that the eye drop preparation was globular in composition and that its distribution became patchy over the cornea with time for both the adult and the pup.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          04 December 2009
          : 19
          : 5
          : 277-284
          Department of Anatomy/Cell Biology, School of Medicine, Wayne State University, Detroit, Mich., USA
          265507 Ophthalmic Res 1987;19:277–284
          © 1987 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 30 December 1986
          : 23 May 1987
          Page count
          Pages: 8
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Scanning microscopy,Ocular mucus,Sodium hyaluronate,Transmission microscopy,Cornea,Mouse


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