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Contribuciones de la técnica de la Reacción en Cadenas de la Polimerasa a la Epidemiología Molecular de las enfermedades infecciosas en Cuba Translated title: Contributions of the Polymerase Chains Reaction technique to the Molecular Epidemiology of the infectious diseases in Cuba

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      A dictionary of epidemiology

       M Porta,  J Last,  JM Last (2000)
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        Molecular epidemiology of tuberculosis in Cuba outside of Havana, July 1994-June 1995: utility of spoligotyping versus IS6110 restriction fragment length polymorphism.

        Molecular typing has become an important tool for examining the extent of active transmission of tuberculosis. To examine transmission of tuberculosis in Cuba using IS6110 restriction fragment length polymorphism (RFLP) typing and to evaluate the utility of spoligotyping. One hundred and sixty Mycobacterium tuberculosis strains isolated over a one year period in Cuba were subjected to RFLP and spoligotyping. Forty-eight percent of the isolates were found in 19 clusters of strains with identical RFLP patterns. In general, cluster sizes were limited, except for two large institutional outbreaks. Age was strongly inversely correlated to clustering. Most streptomycin-resistant isolates were found in clusters. Fifteen spoligotype clusters comprised 78% of the isolates. Significantly different IS6110 RFLP types subdivided 11 spoligotype clusters, whereas none of the IS6110 clusters were subdivided by spoligotyping. Considering the short study period, 48% clustering is high, indicating that recent transmission plays an important role in Cuba. Although resistance is still a minor problem, transmission of streptomycin-resistant strains occurs. The high polymorphism observed with IS6110 RFLP indicates that this marker is useful for future molecular epidemiological studies in Cuba. Spoligotyping appeared less suitable for population-based studies.
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          A dictionary of epidemiology

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            Author and article information

            Affiliations
            [1 ] Universidad de Ciencias Médicas de La Habana
            [2 ] Universidad de Ciencias Médicas de La Habana
            Contributors
            Role: ND
            Role: ND
            Role: ND
            Journal
            rhcm
            Revista Habanera de Ciencias Médicas
            Rev haban cienc méd
            Universidad de Ciencias Médicas de la Habana (La Habana )
            1729-519X
            December 2014
            : 13
            : 6
            : 927-939
            S1729-519X2014000600013

            http://creativecommons.org/licenses/by/4.0/

            Product
            Product Information: SciELO Cuba
            Categories
            HEALTH CARE SCIENCES & SERVICES

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