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      Development and validation of a solid-phase extraction method using anion exchange sorbent for the analysis of cannabinoids in plasma and serum by gas chromatography-mass spectrometry.

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          Abstract

          The aim of this work was to develop and validate a solid-phase extraction (SPE) method for the analysis of cannabinoids with emphasis on a very extensive and effective matrix reduction in order to ensure constant good results in selectivity and sensitivity regardless of the applied measuring technology. This was obtained by the use of an anion exchange sorbent (AXS) and the purposive ionic interaction between matrix components and this sorbent material. In a first step, the neutral cannabinoids ∆9-tetrahydrocannabinol (THC) and 11-hydroxy-∆9-tetrahydrocannabinol (11-OH-THC) were eluted, leaving 11-nor-9-carboxy-∆9-tetrahydrocannabinol (THC-COOH) and the main interfering matrix components bound to the AXS. In a second step, exploiting differences in pH and polarity, it was possible to separate matrix components and THC-COOH, thereby yielding a clean elution of THC-COOH into the same collecting tube as THC and 11-OH-THC. Even when using a simple measuring technology like gas chromatography with single quadrupole mass spectrometry, this two-step elution allows for an obvious decrease in number and intensity of matrix interference in the chromatogram. Hence, in both plasma and serum, the AXS extracts resulted in very good selectivity. Limits of detection and limits of quantification were below 0.25 and 0.35 ng/mL for the neutral cannabinoids in both matrices, 2.0 and 3.0 ng/mL in plasma and 1.6 and 3.3 ng/mL in serum for THC-COOH. The recoveries were ≥79.8 % for all analytes. Interday and intraday imprecisions ranged from 0.8 to 6.1 % relative standard deviation, and accuracy bias ranged from -12.6 to 3.6 %.

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          Author and article information

          Journal
          Int J Legal Med
          International journal of legal medicine
          Springer Science and Business Media LLC
          1437-1596
          0937-9827
          Jul 2016
          : 130
          : 4
          Affiliations
          [1 ] Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, Münster, 48149, Germany. angela.gasse@ukmuenster.de.
          [2 ] Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, Münster, 48149, Germany.
          Article
          10.1007/s00414-016-1368-6
          10.1007/s00414-016-1368-6
          27072011
          e4baec6a-3bec-44ed-a8eb-6350f735484c
          History

          Cannabinoids,GC-EI-MS,SPE,Anion exchange sorbent
          Cannabinoids, GC-EI-MS, SPE, Anion exchange sorbent

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