Isolation of a New PAK1 Gene from Sea Cucumber ( Apostichopus japonicus) and Its Expression Analysis and Function Characterization

Abstract

The p21-activated kinase 1 (PAK1) is a downstream serine/threonine kinase effector of Rac1/Cdc42 that regulates various biological processes including those associating with pathological inflammation. To investigate the function of PAK1 in echinoderms, we isolated a new PAK1 from sea cucumber Apostichopus japonicus ( AjPAK1) by transcriptome database mining and with rapid amplification of cDNA ends (RACE). The full-length cDNA AjPAK1 was 2303 bp in length, containing a 1587 bp ORF encoding 528 amino acid residues. The deduced AjPAK1 contained a p21-Rho-binding domain (PBD) and a serine/threonine protein kinase catalytic domain (S_TKc), which was similar to the PAK1 of crown-of-thorns starfish Acanthaster planci and other eukaryotes. AjPAK1 expressed in all tissues of adult A. japonicus analyzed with the highest transcript anumdance detected in coelomocytes. Significant change in AjPAK1 abundance was observed at 4, 24, 48 and 72 h after Vibrio splendidus infection. Silencing AjPAK1 induced a significant reduction of lysozyme content in coelomic fluid and relative transcript abundances of AjRac1 and AjMKK3/6 in A. japonicus coelomocytes. These results should aid to characterize PAK1 of sea cucumber and decipher its immune function.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 24 August 2019

Publication date (Print): 01 October 2019

Volume: 18

Issue: 5

Pages: 1147-1157

Affiliations

¹Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea of Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China

²State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China

³The University of New South Wales, Sydney, New South Wales 2052, Australia

Author notes

*Corresponding author: ZHAN Yaoyao

Article

Publisher ID: s11802-019-4034-z

DOI: 10.1007/s11802-019-4034-z

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802