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      Isolation of a New PAK1 Gene from Sea Cucumber ( Apostichopus japonicus) and Its Expression Analysis and Function Characterization

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          Abstract

          The p21-activated kinase 1 (PAK1) is a downstream serine/threonine kinase effector of Rac1/Cdc42 that regulates various biological processes including those associating with pathological inflammation. To investigate the function of PAK1 in echinoderms, we isolated a new PAK1 from sea cucumber Apostichopus japonicus ( AjPAK1) by transcriptome database mining and with rapid amplification of cDNA ends (RACE). The full-length cDNA AjPAK1 was 2303 bp in length, containing a 1587 bp ORF encoding 528 amino acid residues. The deduced AjPAK1 contained a p21-Rho-binding domain (PBD) and a serine/threonine protein kinase catalytic domain (S_TKc), which was similar to the PAK1 of crown-of-thorns starfish Acanthaster planci and other eukaryotes. AjPAK1 expressed in all tissues of adult A. japonicus analyzed with the highest transcript anumdance detected in coelomocytes. Significant change in AjPAK1 abundance was observed at 4, 24, 48 and 72 h after Vibrio splendidus infection. Silencing AjPAK1 induced a significant reduction of lysozyme content in coelomic fluid and relative transcript abundances of AjRac1 and AjMKK3/6 in A. japonicus coelomocytes. These results should aid to characterize PAK1 of sea cucumber and decipher its immune function.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          24 August 2019
          01 October 2019
          : 18
          : 5
          : 1147-1157
          Affiliations
          1Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea of Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China
          2State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China
          3The University of New South Wales, Sydney, New South Wales 2052, Australia
          Author notes
          *Corresponding author: ZHAN Yaoyao
          Article
          s11802-019-4034-z
          10.1007/s11802-019-4034-z
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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