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      Functional and quantitative proteomics using SILAC.

      Nature reviews. Molecular cell biology

      Amino Acids, analysis, metabolism, Animals, Humans, Isotope Labeling, methods, Mass Spectrometry, Protein Interaction Mapping, Proteins, chemistry, Proteomics

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          Abstract

          Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.

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          Journal
          17139335
          10.1038/nrm2067

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