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      ANALISIS DEL CARIOTIPO Y DETECCIÓN DE LOS GENES 5S y 18S/25S rDNA EN CHAETANTHERA MICROPHYLLA (CASS.) HOOK. ET ARN. (ASTERACEAE) Translated title: KAYYOTYPE ANALYSIS AND DETECTION OF 5S AND 18S/25S rDNA GENE SEQUENCES IN CHAETANTHERA MICROPHYLLA (CASS.) HOOK. ET ARN. (ASTERACEAE)

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          Translated abstract

          Double fluorescence in situ hybridization was used to examine the karyotype of Chaetanthera microphylla (Cass.) Hook et Arn. from Chile, including the location of 5S and 18S/25S rDNA gene sequences. The species is 2n = 24, with 8m + 4st chromosomes. Signals of 5S and 18/25S rDNA were seen in two of the 12 chromosome pairs: n° 2 and 6

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          Chromosomal evolution in higher plants

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            Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals

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              Quantitative karyotyping and dual-color FISH mapping of 5S and 18S-25S rDNA probes in the cultivated Phaseolus species (Leguminosae)

              Double-color fluorescence in situ hybridization (FISH) followed by DAPI counterstaining allowed the chromosomal assignment of 5S and 18S-25S rRNA genes in the four cultivated Phaseolus Species; P. vulgaris, P. coccineus, P. acutifolius, and P. lunatus (all: 2n = 2x = 22). The rRNA gene loci display variation between species as reflected in differences of signal size and (or) number. From one to three pairs of 5S sites and one to seven pairs of 18S-25S sites were found in the diploid complements of the four taxa studied. Intraspecific variation was studied in P. vulgaris, and it is shown that the number of 18S-25S rDNA sites differs between cultivars. Cytogenetic mapping was complemented by karyotype analyses. Each of the four cultivated Phaseolus species exhibits a characteristic heterochromatin endowment, with P. acutifolius var. latifolius having the highest amount of C-band material. Quantitative karyotyping in combination with cytogenetic mapping allowed the identification of homeologous chromosomes in the different species.
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                Author and article information

                Journal
                gbot
                Gayana. Botánica
                Gayana Bot.
                Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción (Concepción, , Chile )
                0016-5301
                0717-6643
                2005
                : 62
                : 1
                : 47-49
                Affiliations
                [02] Quedlinburg orgnameInstitute of Horticultural Crops orgdiv1Federal Centre for Breeding Research on Cultivated Plants Germany
                [01] Concepción orgnameUniversidad de Concepción orgdiv1Departamento de Botánica Chile cbaeza@ 123456udec.cl
                Article
                S0717-66432005000100008 S0717-6643(05)06200108
                10.4067/S0717-66432005000100008
                e586e115-d4c3-4551-b100-4c86a3e0161c

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 22 February 2005
                : 09 April 2005
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 19, Pages: 3
                Product

                SciELO Chile

                Categories
                COMUNICACIONES BREVES

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