Seleno-detergent MAD phasing of leukotriene C ₄ synthase in complex with dodecyl-β-d-selenomaltoside

Abstract

Dodecyl-β- d-selenomaltoside in a leukotriene C ₄ synthase crystal exhibited sufficient anomalous diffraction for multiwavelength anomalous diffraction phasing.

Abstract

Dodecyl-β- d-selenomaltoside (SeDDM) is a seleno-detergent with a β-glycosidic seleno-ether in place of the ether moiety in dodecyl-β- d-maltoside. Seleno-detergents are candidates for heavy-atom agents in experimental phasing of membrane proteins in protein crystallography. Crystals of a nuclear membrane-embedded enzyme, leukotriene C ₄ synthase (LTC ₄S), in complex with SeDDM were prepared and a multiwavelength anomalous diffraction (MAD) experiment was performed. The SeDDM in the LTC ₄S crystal exhibited sufficient anomalous diffraction for determination of the structure using MAD phasing.