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      Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile

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          ABSTRACT

          Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.

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          Author and article information

          Contributors
          Role: Editor
          Journal
          J Clin Microbiol
          J. Clin. Microbiol
          jcm
          jcm
          JCM
          Journal of Clinical Microbiology
          American Society for Microbiology (1752 N St., N.W., Washington, DC )
          0095-1137
          1098-660X
          5 April 2017
          23 May 2017
          June 2017
          : 55
          : 6
          : 1894-1901
          Affiliations
          [a ]Central Virology Laboratory, Ministry of Health, Ramat-Gan, Israel
          [b ]Institute of Public Health, Ostrava, Czech Republic
          [c ]University of Defence, Faculty of Military Health Sciences, Hradec Králové, Czech Republic
          [d ]Istituto Superiore di Sanità, Rome, Italy
          [e ]Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
          [f ]University Medical Centre Hamburg-Eppendorf, Department of Medicine, Division of Infectious Diseases and Tropical Medicine, Hamburg, Germany
          [g ]Laboratory of Virology, School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, Chile
          [h ]Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
          [i ]Institute of Tropical & Travel Medicine, Sheba Medical Center, Tel Hashomer, Ramat-Gan, Israel
          Boston Children's Hospital
          Author notes
          Address correspondence to Yaniv Lustig, yaniv.lustig@ 123456sheba.health.gov.il .

          Citation Lustig Y, Zelena H, Venturi G, Van Esbroeck M, Rothe C, Perret C, Koren R, Katz-Likvornik S, Mendelson E, Schwartz E. 2017. Sensitivity and kinetics of an NS1-based Zika virus enzyme-linked immunosorbent assay in Zika virus-infected travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile. J Clin Microbiol 55:1894–1901. https://doi.org/10.1128/JCM.00346-17.

          Article
          PMC5442546 PMC5442546 5442546 00346-17
          10.1128/JCM.00346-17
          5442546
          28381608
          e63bcbf3-1f0e-420f-99ef-d35b30d800d5
          Copyright © 2017 American Society for Microbiology.

          All Rights Reserved.

          History
          : 27 February 2017
          : 17 March 2017
          : 30 March 2017
          Page count
          Figures: 2, Tables: 1, Equations: 0, References: 18, Pages: 8, Words: 4680
          Categories
          Virology
          Custom metadata
          June 2017
          free

          travelers,sensitivity,Zika,NS1,ELISA
          travelers, sensitivity, Zika, NS1, ELISA

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