3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Implications of surface passivation on physicochemical and bioimaging properties of carbon dots

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The prevalence of surface functionalized carbon dots (CDs) with intriguing fluorescence properties has given a new dimension to the field of bioimaging and is perceived as a promising alternative to quantum dots (QDs).

          Abstract

          The prevalence of surface functionalized carbon dots (CDs) with intriguing fluorescence properties has given a new dimension to the field of bioimaging and is perceived as a promising alternative to quantum dots (QDs). In the present work, polyethylene glycol (PEG) and polyethyleneimine (PEI) passivated CDs have been synthesized by one-step hydrothermal carbonization of chitosan. We have made a comparative analysis of the physicochemical and bioimaging properties of PEI based carbon dots (CD-PEI) and PEG based carbon dots (CD-PEG). This article further provides an insight into the role of surface functionality in controlling the bioimaging efficiencies of CDs. The concentration dependent cytotoxic effects of CD-PEI and CD-PEG were studied on normal (BHK-21) and cancer (A549) cell lines and we explored the competitive performance of CD-PEI compared to CD-PEG for bio-applications.

          Related collections

          Most cited references20

          • Record: found
          • Abstract: found
          • Article: not found

          Nano-carrier for gene delivery and bioimaging based on carbon dots with PEI-passivation enhanced fluorescence.

          Polyethylenimine (PEI) functionalized carbon dots (CD-PEI) were fabricated by one-step microwave assisted pyrolysis of glycerol and branched PEI25k mixture where the formation of carbon nanoparticles and the surface passivation were accomplished simultaneously. In this hybrid C-dot, PEI molecule played two key roles in the system - as a nitrogen-rich compound to passivate surface to enhance the fluorescence and as a polyelectrolyte to condense DNA. This CD-PEI was shown to be water soluble and emit stable bright multicolor fluorescence relying on excitation wavelength. The DNA condensation capability and cytotoxicity of CD-PEI could be regulated by pyrolysis time possibly due to the somewhat destruction of PEI during the formation of carbon dots. CD-PEI obtained at an appropriate pyrolysis time exhibited lower toxicity, higher or comparable gene expression of plasmid DNA in COS-7 cells and HepG2 cells relative to control PEI25k. Intriguingly, the CD-PEIs internalized into cells displayed tunable fluorescent emission under varying excitation wavelength, suggesting the potential application of CD-PEI in gene delivery and bioimaging. Copyright © 2012 Elsevier Ltd. All rights reserved.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Carbon “quantum” dots for optical bioimaging

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              One-pot green synthesis of optically pH-sensitive carbon dots with upconversion luminescence.

              One-pot fabrication of fluorescent carbon dots was facilely developed by directly heating ascorbic acid aqueous solution at 90 °C. The resulting carbon dots possess excitation, pH and polarity-dependent luminescence and upconversion fluorescence properties.
                Bookmark

                Author and article information

                Journal
                RSCACL
                RSC Adv.
                RSC Adv.
                Royal Society of Chemistry (RSC)
                2046-2069
                2014
                2014
                : 4
                : 40
                : 20915-20921
                Affiliations
                [1 ]Nanobiotechnology Laboratory
                [2 ]Centre for Nanotechnology
                [3 ]Indian Institute of Technology Roorkee
                [4 ]Roorkee, India
                [5 ]Department of Biotechnology
                Article
                10.1039/C4RA02017K
                e694bcdd-ba3d-426d-b131-9dddafb1df22
                © 2014
                History

                Comments

                Comment on this article