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      Laser capture microdissection of cells from plant tissues.

      Plant physiology

      DNA, Plant, genetics, metabolism, Gene Expression Profiling, Histocytological Preparation Techniques, instrumentation, methods, Lasers, Plant Cells, Plant Proteins, Plants, chemistry, Tissue Fixation, RNA, Plant, Sensitivity and Specificity, Tissue Embedding

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          Abstract

          Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types.

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          Author and article information

          Journal
          12746508
          1540312
          10.1104/pp.102.018127

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