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      Detection and quantification of apoptosis in transiently transfected adherent cells.

      1 ,

      Nucleic acids research

      Oxford University Press (OUP)

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          Abstract

          Apoptosis is a highly conserved form of cell death present in all eukaryotic cell types and controlled by multiple genes. Several methods have been developed to quantify apoptosis, but none is adapted for all cell types. It is particularly difficult to reliably assay apoptosis of adherent cells. We describe a new, rapid and reliable flow cytometric method which can be used for quantifiying apoptosis in a sub-population of transiently transfected adherent cells. This technique is based on the detection of transfected cells and the apoptotic sub-population by immunofluorescence and Annexin-V labelling, respectively.

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          Author and article information

          Journal
          Nucleic Acids Res
          Nucleic acids research
          Oxford University Press (OUP)
          0305-1048
          0305-1048
          Nov 15 1998
          : 26
          : 22
          Affiliations
          [1 ] Institut de Génétique Moléculaire, CNRS UMR 5535, 1919 route de Mende, 34293 Montpellier Cedex 5, France. lassus@jones.cnrs-mop.fr
          Article
          gkb793
          10.1093/nar/26.22.5233
          147970
          9801327
          e6c853ca-8f76-41a0-b3a9-61e0090bbcc0

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