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An Ectopic Imaging Window for Intravital Imaging of Engineered Bone Tissue

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      ABSTRACT

      Tissue engineering is a promising branch of regenerative medicine, but its clinical application remains limited because thorough knowledge of the in vivo repair processes in these engineered implants is limited. Common techniques to study the different phases of bone repair in mice are destructive and thus not optimal to gain insight into the dynamics of this process. Instead, multiphoton‐intravital microscopy (MP‐IVM) allows visualization of (sub)cellular processes at high resolution and frequency over extended periods of time when combined with an imaging window that permits optical access to implants in vivo. In this study, we have developed and validated an ectopic imaging window that can be placed over a tissue‐engineered construct implanted in mice. This approach did not interfere with the biological processes of bone regeneration taking place in these implants, as evidenced by histological and micro–computed tomography (μCT)‐based comparison to control ectopic implants. The ectopic imaging window permitted tracking of individual cells over several days in vivo. Furthermore, the use of fluorescent reporters allowed visualization of the onset of angiogenesis and osteogenesis in these constructs. Taken together, this novel imaging window will facilitate further analysis of the spatiotemporal regulation of cellular processes in bone tissue–engineered implants and provides a powerful tool to enhance the therapeutic potential of bone tissue engineering. © 2017 The Authors JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

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      Most cited references 38

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            Author and article information

            Affiliations
            [ 1 ] Laboratory of Clinical and Experimental Endocrinology Department of Chronic Diseases, Metabolism and Ageing KU Leuven Leuven Belgium
            [ 2 ] Prometheus Division of Skeletal Tissue Engineering KU Leuven Leuven Belgium
            Author notes
            [* ] Address correspondence to: Geert Carmeliet, MD, PhD, Laboratory of Clinical and Experimental Endocrinology, Herestraat 49 bus 902, 3000 Leuven, Belgium. E‐mail: geert.carmeliet@ 123456kuleuven.be

            Contributors
            geert.carmeliet@kuleuven.be
            Journal
            JBMR Plus
            JBMR Plus
            10.1002/(ISSN)2473-4039
            JBM4
            JBMR Plus
            John Wiley and Sons Inc. (Hoboken )
            2473-4039
            31 January 2018
            March 2018
            : 2
            : 2 ( doiID: 10.1002/jbm4.v2.2 )
            : 92-102
            6124161
            10.1002/jbm4.10028
            JBM410028
            © 2017 The Authors JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

            This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

            Counts
            Figures: 5, Tables: 0, Pages: 11, Words: 6154
            Product
            Funding
            Funded by: Scientific Research‐Flanders
            Award ID: G.0A7213 and G.096414
            Funded by: BOF‐KU Leuven GOA
            Award ID: 3M120209
            Funded by: Hercules Foundation
            Award ID: AKUL/11/033
            Categories
            Original Article
            Original Articles
            Custom metadata
            2.0
            jbm410028
            March 2018
            Converter:WILEY_ML3GV2_TO_NLMPMC version:version=5.4.7.1 mode:remove_FC converted:05.09.2018

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