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      Modulation of Aortic and Cardiac G Protein Alpha Subunits and Their mRNAs during Norepinephrine Infusion in Rats

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          Abstract

          These studies examine the possibility that alterations in the expression of G protein α subunits occur during desensitization of adrenergic responses in the cardiovascular system. To desensitize adrenergic receptors, rats were infused with norepinephrine (NE) subcutaneously (0.1 mg/kg/h) for 3 or 6 days using osmotic minipumps. G protein α subunits and their mRNAs were then measured in the aorta and heart using selective antibodies and cDNA probes. Infusion of NE for 6 days significantly decreases the levels of G<sub>sα</sub>, G<sub>iα</sub> and G<sub>oα</sub> in the aorta. The mRNAs for the α subunits are not altered in the aorta after NE infusion for 3 or 6 days indicating that reduced mRNA expression does not account for the decreased proteins. In the atrium and ventricle the levels of G<sub>sα </sub>decrease after NE infusion for 3 days but then return to control levels by day 6. The levels of atrial and ventricular G<sub>iα</sub> are unaltered after NE infusion for 3 days but increase significantly by day 6. G<sub>oα</sub> levels do not change in the atrium or ventricle on either day. The level of G<sub>i2α</sub> mRNA increases after NE infusion for 6 days and may account for the increased αi protein. The levels of the other G<sub>α</sub> mRNAs do not change in the atrium or ventricle. These results demonstrate that expression of G protein α subunits is altered during cardiovascular desensitization, raising the possibility that modulation of the α subunits may contribute to reduced adrenergic responsiveness.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1995
          1995
          24 September 2008
          : 32
          : 1
          : 16-23
          Affiliations
          Departments of aPharmacology, and bPsychiatry, Medical College of Pennsylvania, Philadelphia, Pa., USA
          Article
          159073 J Vasc Res 1995;32:16–23
          10.1159/000159073
          7873705
          © 1995 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 8
          Categories
          Research Paper

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