Delivery of macromolecules into the endothelium of whole ex vivo human cornea by femtosecond laser-activated carbon nanoparticles.

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Abstract

The targeted delivery of drugs or genes into corneal endothelial cells (ECs) during eye banking could help improve graft quality and quantity. Physical methods raising less safety concerns than viral ones, we previously adapted, for in vitro ECs, a recent innovative technique of drug delivery based on the activation of carbon nanoparticles (CNPs) by a femtosecond laser (fsL). The aim of the present pilot study was to adapt this method to enable molecule delivery into the intact endothelium of ex vivo human corneas.

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Author and article information

Journal

Journal ID (iso-abbrev): Br J Ophthalmol

Title: The British journal of ophthalmology

Publisher: BMJ

ISSN (Electronic): 1468-2079

ISSN (Print): 0007-1161

Publication date (Electronic): August 2016

Volume: 100

Issue: 8

Affiliations

[1 ] Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, Saint-Etienne, France.

[2 ] Hubert Curien Laboratory, UMR-CNRS 5516, Jean Monnet University, Saint-Etienne, France Manutech-USD, Saint-Etienne, France.

[3 ] Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, Saint-Etienne, France Ophthalmology Department, University Hospital, Saint-Etienne, France.

[4 ] Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, Saint-Etienne, France Ophthalmology Department, University Hospital, Saint-Etienne, France Institut Universitaire de France, Paris, France.

Article

Publisher Item ID: bjophthalmol-2015-307610

DOI: 10.1136/bjophthalmol-2015-307610

PubMed ID: 27226345

SO-VID: e730ef54-6ddb-4dc7-a2c6-50c24f7852aa

History

Keywords: Drugs,Experimental &#8211 laboratory,Treatment Lasers,Cornea

Data availability:

Keywords: Drugs, Experimental &#8211 laboratory, Treatment Lasers, Cornea

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