On the Measurement of Vascular and Respiratory Smooth Muscle Responses  in vitro

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Abstract

The action of drugs on circular smooth muscle from rabbit renal artery, renal vein, mesenteric vein, inferior vena cava, guinea pig bronchus, and mouse trachea was studied using an inexpensive modification of the method of Bevan, Nielsen and Ovvman, and Bevan and Osher. Two 30-gauge disposable stainless steel hypodermic needles supported the tissue rings in a standard 10-ml isolated muscle bath. All the blood vessels contracted to norepinephrine, histamine, and KCl. The veins, unlike the renal artery, did not respond maximally to KCl and did not contract at all to serotonin. The guinea pig bronchi contracted to carbachol, histamine, and KCl and relaxed to isoproterenol. The mouse trachea contracted maximally to carbachol, did not contract to histamine, and contracted to 50% of maximum in response to KCl. Thus, standard isolated tissue baths can be inexpensively adapted for the study of relatively small blood vessels and airways (about 1 mm in diameter).

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Author and article information

Journal

Journal ID (publisher-id): JVR

Journal ID (nlm-ta): J Vasc Res

Journal ID (doi): 10.1159/issn.1018-1172

Title: Journal of Vascular Research

Publisher: S. Karger AG

ISSN (Print): 1018-1172

ISSN (Electronic): 1423-0135

Publication date Subscription-year: 1977

Publication date (Print): 1977

Publication date (Electronic): 18 September 2008

Volume: 14

Issue: 1

Pages: 1-11

Affiliations

Division of Pharmacological Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Ind.

Article

Publisher ID: 158110 Other ID: Blood Vessels 1977;14:1–11

DOI: 10.1159/000158110

SO-VID: e78ce58b-fdaa-43c5-a028-600a56eb2dc6

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 04 December 1975

Date accepted : 23 April 1976

Page count

Pages: 11

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On the Measurement of Vascular and Respiratory Smooth Muscle Responses in vitro

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