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      Screening and Characterization of Nitrite-Degrading Bacterial Isolates Using a Novel Culture Medium

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          Abstract

          In this study, a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates. The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the major carbon source, to achieve the high nitrogen and low carbon nutritional status found in shrimp farming ponds. Screening using this medium identified potent denitrifying Bacillus isolates, among which Bacillus subtilis M7-1 was considered best. M7-1 was able to completely degrade nitrite-N in 24 h without much consumption of dissolved oxygen. Efficient denitrification activity took place in liquid cultures within a set of non-stringent ranges of pH (5.0–9.0), salinity (0–30) and temperature (25–35°C). The denitrifying enzyme gene was amplified, sequenced and further identified as nirS type. In biosecurity assessments, M7-1 had no negative effects on shrimps at a dose of 10 6 cfu mL −1. M7-1 could therefore be used in aquaculture to reduce and control the nitrogen concentration, and to promote the development of sustainable and healthy culture systems.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          20 December 2019
          01 February 2020
          : 19
          : 1
          : 241-248
          Affiliations
          1School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
          2Marine Biomedical Research Institute of Qingdao, Qingdao 266003, China
          Author notes
          *Corresponding author: HE Zengguo, Tel: 0086-532-85906865, E-mail: bioantai88@ 123456vip.163.com
          Article
          s11802-020-4093-1
          10.1007/s11802-020-4093-1
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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