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      Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-κB signaling pathway.

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          Abstract

          Bupleurum chinense is a well-known traditional Chinese medicine. Polysaccharides extracted from medical plants possess multiple healthy benefits. In the present study, an alkali-extracted polysaccharide (BCAP-1) was isolated from Bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. BCAP-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by DEAE-cellulose and Sepharose CL-6B columns. BCAP-1 markedly inhibited Sarcoma 180tumor growth in tumor-bearing mice, and increased the secretion of TNF-α in serum. MTT assay showed that BCAP-1 had no cytotoxicity against S-180 tumor cells. BCAP-1 enhanced the secretion of TNF-α and NO, and the transcripts of TNF-α and iNOS were increased. Meanwhile, BCAP-1 treatment induced the phosphorylation of p65 and decreased the expression of IκB in macrophages. These results suggest that BCAP-1 could activate macrophages through NF-κB signaling pathway, and the anti-tumor effects of BCAP-1 can be achieved by its immunostimulating features.

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          Author and article information

          Journal
          Int. J. Biol. Macromol.
          International journal of biological macromolecules
          Elsevier BV
          1879-0003
          0141-8130
          Feb 2017
          : 95
          Affiliations
          [1 ] School of Public Health, Jilin University, Changchun 130021, China.
          [2 ] Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China.
          [3 ] School of Life Science, Jilin Agricultural University, Changchun 130118, China.
          [4 ] School of Public Health, Jilin University, Changchun 130021, China. Electronic address: zhicheng@jlu.edu.cn.
          [5 ] College of Forestry, Beihua University, Jilin 132013, China. Electronic address: dfg4656@hotmail.com.
          [6 ] Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China. Electronic address: tonghaibin@gmail.com.
          Article
          S0141-8130(16)31290-9
          10.1016/j.ijbiomac.2016.10.112
          27884671

          Bupleurum chinense, Immunomodulation, Polysaccharide

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