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      Investigations into the Effect of Glyoxylate Decarboxylation and Transamination on Oxalate Formation in the Rat

      a , a , b


      S. Karger AG

      Hyperoxaluria, Glyoxylate, Decarboxylation, Transamination, Cysteine

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          The decarboxylation and transamination reactions of glyoxylate, which divert this precursor from oxalate formation, have been investigated. Decarboxylation of glyoxylate is synergistic with 2-oxoglutarate and catalysed by 2-oxoglutarate :glyoxylate carboligase which co-chromatographs with the 2-oxoglutarate dehydrogenase complex. The activity is located in the mitochondrial fraction and is probably due to the E<sub>1</sub> subunit of the complex. A greater amount of decarboxylation occurs from 2-oxoglutarate than from glyoxylate but the presence of 2-oxoglutarate does not affect oxalate formation from glyoxylate. There is no oxalate formation from 2-oxoglutarate. Studies with rat liver homogenates showed that a number of amino acids can participate in glyoxylate transamination. However, using isolated rat hepatocytes, these reactions did not have a significant effect on oxalate formation from glyoxylate with the exception of cysteine which caused an 80% reduction in oxalate formation. Investigation of this inhibition indicated that it was most likely due to the formation of a cysteine-glyoxylate adduct which makes glyoxylate unavailable for oxidation to oxalate. This cysteine inhibition of oxalate formation was also demonstrated in normal rats and rats made hyperoxaluric by injecting them with either glyoxylate or glycolate. The results indicate that sulphydryl compounds, which can have a therapeutic role as oxalate-lowering agents, may be able to be developed.

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          Author and article information

          S. Karger AG
          11 December 2008
          : 57
          : 4
          : 460-469
          aDivision of Clinical Chemistry, Institute of Medical and Veterinary Science, Adelaide.; bDepartment of Biochemistry, Alfred Hospital, Melbourne, Australia
          186350 Nephron 1991;57:460–469
          © 1991 S. Karger AG, Basel

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          Page count
          Pages: 10
          Original Paper

          Cardiovascular Medicine, Nephrology

          Hyperoxaluria, Glyoxylate, Decarboxylation, Transamination, Cysteine


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