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      [The fluorescent staining of mitochondria in living HeLa- and LM-cells with new acridine dyes (author's transl)].

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          Abstract

          The fluorescent staining of mitochondria in living cells with new acridine dyes is reported. The fluorescent dyes used are derivatives of acridine orange (AO) and of 3-amino-6-methoxyacridine (AMA) with various residues in 9- or 10-position (Scheme 1). They are either permanent cationic dyes or cations which are formed by protonation in the culture medium. HeLa cells and mouse fibroblasts (LM cells) have been used for our staining experiments. On favourable conditions we succeeded in staining the mitochondria not only orthochromatically but also metachromatically. Photodynamical effects which have been observed during the exposure of the stained cells in the fluorescence microscope are described. The residues in 9- or 10-position favour the dye accumulation in the mitochondria. Vital staining with the basic compounds AO and AMA however leads to the formation of metachromatically stained lysosomes in the orthochromatically stained cytoplasm. The dye 3-amino-6-methoxy-9-(2-hydroxyethyl)acridine stains the nucleus of living cells.

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          Author and article information

          Journal
          Histochemistry
          Histochemistry
          Springer Science and Business Media LLC
          0301-5564
          0301-5564
          1982
          : 74
          : 1
          Article
          10.1007/BF00495046
          7085344
          e7d63a95-0e2a-40c0-99d7-7f0b4a3c4979
          History

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