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      Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads

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          Abstract

          Whole saliva (WS) was used for diagnosis of oral squamous cell carcinoma (OSCC); two polypeptides may be used for OSCC diagnosis.

          Abstract

          Saliva diagnostics utilizing nanotechnology and molecular technologies to detect oral squamous cell carcinoma (OSCC) has become an attractive field of study. However, no specific methods have been established. To refine the diagnostic power of saliva peptide fingerprints for the early detection of OSCC, we screened the expression spectrum of salivary peptides in 40 T1 stage OSCC patients (and healthy controls) using MALDI-TOF-MS combined with magnetic beads. Fifty proteins showed significantly different expression levels in the OSCC samples ( P<0.05). Potential biomarkers were also predicted. The novel diagnostic proteomic model with m/z peaks of 1285.6 Da and 1432.2 Da are of certain value for early diagnosis of OSCC.

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          Global cancer statistics in the year 2000.

          D Parkin (2001)
          Estimation of the burden of cancer in terms of incidence, mortality, and prevalence is a first step to appreciating appropriate control measures in a global context. The latest results of such an exercise, based on the most recent available international data, show that there were 10 million new cases, 6 million deaths, and 22 million people living with cancer in 2000. The most common cancers in terms of new cases were lung (1.2 million), breast (1.05 million), colorectal (945,000), stomach (876,000), and liver (564,000). The profile varies greatly in different populations, and the evidence suggests that this variation is mainly a consequence of different lifestyle and environmental factors, which should be amenable to preventive interventions. World population growth and ageing imply a progressive increase in the cancer burden--15 million new cases and 10 million new deaths are expected in 2020, even if current rates remain unchanged.
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            Virulence factors of Candida albicans

            Richard A. Calderone, William Fonzi (2001)
            Candidiasis is a common infection of the skin, oral cavity and esophagus, gastrointestinal tract, vagina and vascular system of humans. Although most infections occur in patients who are immunocompromised or debilitated in some other way, the organism most often responsible for disease, Candida albicans, expresses several virulence factors that contribute to pathogenesis. These factors include host recognition biomolecules (adhesins), morphogenesis (the reversible transition between unicellular yeast cells and filamentous, growth forms), secreted aspartyl proteases and phospholipases. Additionally, 'phenotypic switching' is accompanied by changes in antigen expression, colony morphology and tissue affinities in C. albicans and several other Candida spp. Switching might provide cells with a flexibility that results in the adaptation of the organism to the hostile conditions imposed not only by the host but also by the physician treating the infection.
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              The salivary microbiota as a diagnostic indicator of oral cancer: A descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects

              DL Mager, AD Haffajee, PM Devlin … (2005)
              Background The purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (OSCC) lesion would differ from those found in cancer-free (OSCC-free) controls. Methods Unstimulated saliva samples were collected from 229 OSCC-free and 45 OSCC subjects and evaluated for their content of 40 common oral bacteria using checkerboard DNA-DNA hybridization. DNA counts per ml saliva were determined for each species, averaged across subjects in the 2 subject groups, and significance of differences between groups determined using the Mann-Whitney test and adjusted for multiple comparisons. Diagnostic sensitivity and specificity in detection of OSCC by levels of salivary organisms were computed and comparisons made separately between a non-matched group of 45 OSCC subjects and 229 controls and a group of 45 OSCC subjects and 45 controls matched by age, gender and smoking history. Results Counts of 3 of the 40 species tested, Capnocytophaga gingivalis, Prevotella melaninogenica and Streptococcus mitis, were elevated in the saliva of individuals with OSCC (p < 0.001). When tested as diagnostic markers the 3 species were found to predict 80% of cancer cases (sensitivity) while excluding 83% of controls (specificity) in the non-matched group. Diagnostic sensitivity and specificity in the matched group were 80% and 82% respectively. Conclusion High salivary counts of C. gingivalis, P. melaninogenica and S. mitis may be diagnostic indicators of OSCC.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Biosci Rep
                Journal ID (iso-abbrev): Biosci. Rep
                Journal ID (hwp): ppbioscirep
                Journal ID (publisher-id): BSR
                Title: Bioscience Reports
                Publisher: Portland Press Ltd.
                ISSN (Print): 0144-8463
                ISSN (Electronic): 1573-4935
                Publication date (Electronic preprint): 12 May 2015
                Publication date (Electronic): 22 June 2015
                Publication date Collection: June 2015
                Volume: 35
                Issue: 3 ( displayID: 3 )
                Electronic Location Identifier: e00211
                Affiliations
                [* ]The Third Dental Center, Peking University School and Hospital of Stomatology, Beijing 100083, PR China
                [† ]Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, PR China
                [‡ ]Department of Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, PR China
                Author notes
                [ 1 ]To whom correspondence should be addressed (email moleculecf@ 123456gmail.com ).
                Article
                Publisher ID: e00211
                DOI: 10.1042/BSR20150023
                PMC ID: 4613719
                PubMed ID: 26182373
                SO-VID: e7d7f235-5c94-4252-a9a7-0e9d0e1539f9
                Copyright © © 2015 The Authors
                License:

                This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution License 3.0.

                History
                Date received : 28 January 2015
                Date revision received : 6 May 2015
                Date accepted : 8 May 2015
                Page count
                Figures: 4, Tables: 2, References: 64, Pages: 9
                Categories
                Subject: Original Papers
                Subject: Original Paper

                ScienceOpen disciplines: Life sciences
                Keywords: early diagnosis,histatin-3,matrix-assisted laser-desorption ionization–time-of-flight–mass spectrometry (maldi-tof-ms),oral squamous cell carcinoma (oscc),peptides,saliva
                Data availability:
                ScienceOpen disciplines: Life sciences
                Keywords: early diagnosis, histatin-3, matrix-assisted laser-desorption ionization–time-of-flight–mass spectrometry (maldi-tof-ms), oral squamous cell carcinoma (oscc), peptides, saliva

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