Molecular and morphological evidence of hybridization between two dimorphic sympatric species of Fuchsia (Onagraceae)

Abstract

Hybridization is commonly reported in angiosperms, generally based on morphology, and in few cases confirmed by molecular markers. Fuchsia has a long tradition of ornamental cultivars with different hybrids produced by artificial crosses, so natural hybridization between sympatric Fuchsia species could be common. Natural hybridization between F. microphylla and F. thymifolia was tested using six newly developed microsatellites for F. microphylla in addition to other molecular markers with codominant and maternal inheritance. Geometric morphometrics of leaves and floral structures were also used to identify putative hybrids. Hybrids showed a different degree of genetic admixture between both parental species. Chloroplast DNA (cpDNA) sequences indicated that hybridization occurs in both directions, in fact, some of the hybrids showed new haplotypes for cpDNA and ITS (internal transcriber spacer of nuclear ribosomal RNA genes) sequences. The morphology of hybrid individuals varied between the two parental species, but they could be better identified by their leaves and floral tubes. Our study is the first to confirm the hybridization in natural populations of Fuchsia species and suggests that hybridization has probably occurred repeatedly throughout the entire distribution of the species. Phylogeographic analysis of both species will be essential to understanding the impact of hybridization throughout their complete distribution.

Abstract

Fuchsia is a genus widely used for ornamental purposes, with different hybrids produced by artificial crosses. We confirmed for the first time the hybridization in natural populations of this genus, using molecular markers and geometric morphometrics of leaves and floral structures. The results suggest that hybridization has probably occurred repeatedly throughout the entire distribution of the species and hybridization occurs in both directions with respect to parental species. The morphology of hybrid individuals varied between the two parental species, but they could be better identified by their leaves and floral tubes.