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      Isolation of a major cell surface glycoprotein from fibroblasts.

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          Abstract

          A cell surface component has been isolated in partially purified form from cultured chick embryo and chick heart fibroblasts. This glycoprotein is similar to a protein recently reported to be present at the surface of normal cells, and missing after neoplastic transformation. It is a major cell surface glycoprotein that is synthesized by cultured fibroblasts, but is not collagen. It is shown to be markedly trypsin-sensitive, and its recovery from the cell surface is dependent on cell density. It is excluded from Sephadex G-200, but is not rapidly sedimented by ultracentrifugation, and has an apparent molecular weight of 220,000. The isolation of this cell surface glycoprotein may now provide a means of determining its function.

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          Author and article information

          Journal
          Proc Natl Acad Sci U S A
          Proceedings of the National Academy of Sciences of the United States of America
          Proceedings of the National Academy of Sciences
          0027-8424
          0027-8424
          Sep 1974
          : 71
          : 9
          Article
          10.1073/pnas.71.9.3492
          433800
          4530317
          e8206f00-13ce-4034-a387-41d4a2867c8e
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