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G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein.

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      Abstract

      G-rich is a Drosophila melanogaster selenoprotein, which is a homologue of human and mouse SelK. Subcellular localization analysis using GFP-tagged G-rich showed that G-rich was localized in the Golgi apparatus. The fusion protein was co-localized with the Golgi marker proteins but not with an endoplasmic reticulum (ER) marker protein in Drosophila SL2 cells. Bioinformatic analysis of G-rich suggests that this protein is either type II or type III transmembrane protein. To determine the type of transmembrane protein experimentally, GFP-G-rich in which GFP was tagged at the N-terminus of G-rich, or G-rich-GFP in which GFP was tagged at the C-terminus of G-rich, were expressed in SL2 cells. The tagged proteins were then digested with trypsin, and analyzed by Western blot analysis. The results showed that the C-terminus of the G-rich protein was exposed to the cytoplasm indicating it is a type III microsomal membrane protein. G-rich is the first selenoprotein identified in the Golgi apparatus.

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      Affiliations
      [1 ] School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.
      Journal
      Biochem. Biophys. Res. Commun.
      Biochemical and biophysical research communications
      Elsevier BV
      0006-291X
      0006-291X
      Oct 06 2006
      : 348
      : 4
      16920070
      S0006-291X(06)01742-6
      10.1016/j.bbrc.2006.07.203

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