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      The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements.

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      Nature biotechnology

      Springer Nature

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          Abstract

          Over the last decade, the introduction of microarray technology has had a profound impact on gene expression research. The publication of studies with dissimilar or altogether contradictory results, obtained using different microarray platforms to analyze identical RNA samples, has raised concerns about the reliability of this technology. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and data analysis issues. Expression data on four titration pools from two distinct reference RNA samples were generated at multiple test sites using a variety of microarray-based and alternative technology platforms. Here we describe the experimental design and probe mapping efforts behind the MAQC project. We show intraplatform consistency across test sites as well as a high level of interplatform concordance in terms of genes identified as differentially expressed. This study provides a resource that represents an important first step toward establishing a framework for the use of microarrays in clinical and regulatory settings.

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          Author and article information

          Journal
          Nat. Biotechnol.
          Nature biotechnology
          Springer Nature
          1087-0156
          1087-0156
          Sep 2006
          : 24
          : 9
          Affiliations
          [1 ] National Center for Toxicological Research, US Food and Drug Administration, Jefferson, Arkansas 72079, USA.
          Article
          nbt1239 NIHMS352334
          10.1038/nbt1239
          3272078
          16964229

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