Ding Luo 1 , Xiaonong Fan 2 , 3 , 4 , Congcong Ma 1 , Tongtao Fan 1 , Xiaoguang Wang 1 , Nvzi Chang 1 , Lingxin Li 1 , 5 , Yanan Zhang 2 , 3 , 4 , Zhihong Meng 4 , 6 , Shu Wang 2 , 4 , Xuemin Shi 2 , 4 , 6 , *
10 July 2014
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin +, BrdU + and nestin/BrdU +) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's “self-healing” capacity 3 days after MCAO. And the “self-healing” capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3 β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3 β and PP2A expression in MCAO rats.