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      Neuronal Expression of Tumor Necrosis Factor Alpha in the OVOUJI fme Brain

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          Abstract

          We have examined the expression of the inflammatory cytokine, tumor necrosis factor alpha (TNFα) in the mouse brain. Using immunohistochemical methods developed, we found anti-TNFα immunoreactivity localized in the basal ganglia and other discrete brain structures. Constitutive immunoreactivity, present in normal, unstimulated brain, was observed in glial and microglial-like cells, but it was predominant in neuronal-like cells. Intravenous administration of lipopolysaccharide (LPS) increased TNFα transcript levels detected by RT-PCR in specific brain subregions in which contaminating blood cells were removed. The maximal increase occurred within 2 h of LPS injection; transcripts diminished to near control levels in the next 4 h. Immunocytochemical analysis and single-cell RT-PCR analysis of primary cultures of cortical neuronal cells confirmed expression of TNFα in cells that also express neuronal-specific enolase RNA. Addition of LPS or recombinant TNFα protein to neuronal cultures enhanced expression of TNFα transcripts. Our results indicate that in addition to glial and microglial cells, a well-defined subset of neuronal cells also express TNFα constitutively; this expression can be altered by both extrinsic (LPS) and intrinsic (TNFα itself) factors.

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          Author and article information

          Journal
          NIM
          Neuroimmunomodulation
          10.1159/issn.1021-7401
          Neuroimmunomodulation
          S. Karger AG
          1021-7401
          1423-0216
          1996
          1996
          04 June 1997
          : 3
          : 5
          : 289-303
          Affiliations
          aGeriatric Research Education and Clinical Center, Salt Lake City VA Medical Center, and bDivision of Geriatrics, Department of Medicine, Human Molecular Biology and Genetics Program, and dDepartment of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah, USA
          Article
          97283 Neuroimmunomodulation 1996;3:289–289
          10.1159/000097283
          9218250
          © 1996 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 15
          Categories
          Original Paper

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