The genus Echinoporia is characterized by the presence of chlamydospores originating from hairs in the pileus and margin, or the dissepiment edges. Two species of Echinoporia are found in America, i.e., E. aculeifera and E. inermis. In a study of Neotropical polypores, several specimens of Echinoporia were collected in the Atlantic rainforest, São Paulo state. Echinoporia inermis is recorded for the second time in Brazil. Echinoporia aculeifera is recorded for the first time in São Paulo state. A distribution map of the genus in the Neotropics is presented.