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      A Postsynaptic Role for Rhp55/57 That Is Responsible for Cell Death in Δrqh1 Mutants Following Replication Arrest in Schizosaccharomyces pombe

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      Genetics
      Genetics Society of America

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          Abstract

          Following replication arrest, multiple cellular responses are triggered to maintain genomic integrity. In fission yeast, the RecQ helicase, Rqh1, plays a critical role in this process. This is demonstrated in Deltarqh1 cells that, following treatment with hydroxyurea (HU), undergo an aberrant mitosis leading to cell death. Previous data suggest that Rqh1 functions with homologous recombination (HR) in recovery from replication arrest. We have found that loss of the HR genes rhp55(+) or rhp57(+), but not rhp51(+) or rhp54(+), suppresses the HU sensitivity of Deltarqh1 cells. Much of this suppression requires Rhp51 and Rhp54. In addition, this suppression is partially dependent on swi5(+). In budding yeast, overexpressing Rad51 (the Rhp51 homolog) minimized the need for Rad55/57 (Rhp55/57) in nucleoprotein filament formation. We overexpressed Rhp51 in Schizosaccharomyces pombe and found that it greatly reduced the requirement for Rhp55/57 in recovery from DNA damage. However, overexpressing Rhp51 did not change the Deltarhp55 suppression of the HU sensitivity of Deltarqh1, supporting an Rhp55/57 function during HR independent of nucleoprotein filament formation. These results are consistent with Rqh1 playing a role late in HR following replication arrest and provide evidence for a postsynaptic function for Rhp55/57.

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          Author and article information

          Journal
          Genetics
          Genetics
          Genetics Society of America
          0016-6731
          1943-2631
          June 24 2005
          June 2005
          June 2005
          March 31 2005
          : 170
          : 2
          : 519-531
          Article
          10.1534/genetics.104.037598
          1450410
          15802523
          e9976801-bbe9-4eba-94f3-7d8f89ba7935
          © 2005
          History

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