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      Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.

      Nature Methods

      DNA Primers, chemistry, Genetic Code, RNA, Bacterial, RNA, Ribosomal, 16S, methods, Sequence Analysis, DNA

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          Abstract

          We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

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          Author and article information

          Journal
          18264105
          3439997
          10.1038/nmeth.1184

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