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      Mammalian cell transfection: the present and the future

      research-article
      Author(s): 1 , 1 , 2 ,
      Publication date (Electronic):
      Journal: Analytical and Bioanalytical Chemistry
      Publisher: Springer-Verlag
      Keywords: Transfection, Nucleic acid, Gene, Single cell

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          Abstract

          Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microcope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.

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          The promises and pitfalls of RNA-interference-based therapeutics.

          Daniela Castanotto, John J Rossi (2009)
          The discovery that gene expression can be controlled by the Watson-Crick base-pairing of small RNAs with messenger RNAs containing complementary sequence - a process known as RNA interference - has markedly advanced our understanding of eukaryotic gene regulation and function. The ability of short RNA sequences to modulate gene expression has provided a powerful tool with which to study gene function and is set to revolutionize the treatment of disease. Remarkably, despite being just one decade from its discovery, the phenomenon is already being used therapeutically in human clinical trials, and biotechnology companies that focus on RNA-interference-based therapeutics are already publicly traded.
          Article 0 comments Cited 306 times – based on 0 reviews     Review now
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            Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo.

            F. Scherer, M. Anton, U Schillinger … (2002)
            Low efficiencies of nonviral gene vectors, the receptor-dependent host tropism of adenoviral or low titers of retroviral vectors limit their utility in gene therapy. To overcome these deficiencies, we associated gene vectors with superparamagnetic nanoparticles and targeted gene delivery by application of a magnetic field. This potentiated the efficacy of any vector up to several hundred-fold, allowed reduction of the duration of gene delivery to minutes, extended the host tropism of adenoviral vectors to nonpermissive cells and compensated for low retroviral titer. More importantly, the high transduction efficiency observed in vitro was reproduced in vivo with magnetic field-guided local transfection in the gastrointestinal tract and in blood vessels. Magnetofection provides a novel tool for high throughput gene screening in vitro and can help to overcome fundamental limitations to gene therapy in vivo.
            Article 0 comments Cited 176 times – based on 0 reviews     Review now
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              Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery.

              Annette Dobson (2006)
              The recent emphasis on the development of non-viral transfection agents for gene delivery has led to new physics and chemistry-based techniques, which take advantage of charge interactions and energetic processes. One of these techniques which shows much promise for both in vitro and in vivo transfection involves the use of biocompatible magnetic nanoparticles for gene delivery. In these systems, therapeutic or reporter genes are attached to magnetic nanoparticles, which are then focused to the target site/cells via high-field/high-gradient magnets. The technique promotes rapid transfection and, as more recent work indicates, excellent overall transfection levels as well. The advantages and difficulties associated with magnetic nanoparticle-based transfection will be discussed as will the underlying physical principles, recent studies and potential future applications.
              Article 0 comments Cited 110 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                : +1-215-8980420 , +1-215-5732236 , eberwine@upenn.edu
                Journal
                Journal ID (nlm-ta): Anal Bioanal Chem
                Title: Analytical and Bioanalytical Chemistry
                Publisher: Springer-Verlag (Berlin/Heidelberg )
                ISSN (Print): 1618-2642
                ISSN (Electronic): 1618-2650
                Publication date (Electronic): 13 June 2010
                Publication date PMC-release: 13 June 2010
                Publication date (Print): August 2010
                Volume: 397
                Issue: 8
                Pages: 3173-3178
                Affiliations
                [1 ]Department of Pharmacology, University of Pennsylvania Medical School—Pharmacology, 36th and Hamilton Walk, Philadelphia, PA 19104 USA
                [2 ]Penn Genome Frontiers Institute, University of Pennsylvania Medical School—Pharmacology, 36th and Hamilton Walk, Philadelphia, PA 19104 USA
                Article
                Publisher ID: 3821
                DOI: 10.1007/s00216-010-3821-6
                PMC ID: 2911531
                PubMed ID: 20549496
                SO-VID: e9fdf1e3-56a0-4ece-b2e2-796c5ee190c0
                Copyright © © The Author(s) 2010
                History
                Date received : 9 February 2010
                Date revision received : 28 April 2010
                Date accepted : 3 May 2010
                Categories
                Subject: Trends
                Custom metadata
                issue-copyright-statement © Springer-Verlag 2010

                ScienceOpen disciplines: Analytical chemistry
                Keywords: single cell,transfection,nucleic acid,gene
                Data availability:
                ScienceOpen disciplines: Analytical chemistry
                Keywords: single cell, transfection, nucleic acid, gene

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