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      mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels.

      Science (New York, N.Y.)
      pharmacology, Alternative Splicing, Amino Acid Sequence, Animals, Calcium, metabolism, Charybdotoxin, DNA, genetics, Drosophila, Electric Conductivity, Large-Conductance Calcium-Activated Potassium Channels, Membrane Proteins, chemistry, Mice, Molecular Sequence Data, Oocytes, Peptides, Potassium, Potassium Channels, drug effects, Potassium Channels, Calcium-Activated, RNA, RNA, Complementary, Scorpion Venoms, Sodium, Tetraethylammonium, Tetraethylammonium Compounds, Transcription, Genetic, Xenopus

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          Abstract

          Complementary DNAs (cDNAs) from mSlo, a gene encoding calcium-activated potassium channels, were isolated from mouse brain and skeletal muscle, sequenced, and expressed in Xenopus oocytes. The mSlo-encoded channel resembled "maxi" or BK (high conductance) channel types; single channel conductance was 272 picosiemens with symmetrical potassium concentrations. Whole cell and single channel currents were blocked by charybdotoxin, iberiotoxin, and tetraethylammonium ion. A large number of variant mSlo cDNAs were isolated, indicating that several diverse mammalian BK channel types are produced by a single gene.

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