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Abstract
By using a chromogenic substrate for an ubiquitous lysosomal enzyme, hexosaminidase
to estimate cell numbers, a sensitive and simple procedure has been developed in which
microtiter reaction wells are directly scanned in a spectrophotometer. This method
has been adapted to several cell biological assays. Quantitation of the biological
activities of T cell growth factor and interferon can be performed on large numbers
of samples. Adhesion of dispersed solid tissue cells to fibronectin coated substrates
may be quantitated with little expenditure of reagents. By use of a panning procedure
in microtiter plates a sensitive and very simple assay for the binding of monoclonal
antibodies to cell surface antigens has been developed.