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      Phosphorus-31 NMR Analysis of Dynamic Energy Metabolism in Intact Crystalline Lens Treated with Ouabain: Phosphorylated Metabolites


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          Changes in phosphatic metabolites and intralenticular pH of the intact crystalline rabbit lens induced by ouabain (10<sup>––3</sup>, 10<sup>––4</sup>, 10<sup>––5</sup> M) were measured during time-course incubations using phosphorus-31 nuclear magnetic resonance spectroscopy (<sup>31</sup>P NMR) in order to study the metabolic manifestations of this steroid on lens glycolytic activity. Significant alterations in the glycolytic intermediates of the crystalline lens only occurred with ouabain at the concentration of 10<sup>––3</sup> M. A significant time-dependent increase in α-glycerophosphate, an unidentified pentose phosphate, the nucleoside diphosphosugars (uridine diphosphoglucose and uridine diphosphomannose), inorganic orthophosphate, and inosine monophosphate, and decline in glucose 6-phosphate and ATP were detected in 10<sup>––</sup><sup>3</sup> M ouabain-treated lenses. The uridine diphosphosugars exhibited a maximum at approximately the time when the lens ATP content was 50% of control levels (12.5 h). A progressive decrease in intralenticular pH indicative of hydrogenion-pump damage was detected concomitant with the changes in lens metabolite levels. Lenses maintained transparency throughout the time-course. Since metabolite manifestations effecting the intermediates of glycolysis occur in lens tissue with its high glycolytic and low oxidative activity, we hypothesize the effects of 10<sup>––3</sup> M ouabain may represent metabolic actions of ouabain which are independent of its effects on tissue oxygen consumption as is presumed from studies involving tissues more dependent on oxidative metabolism.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          04 December 2009
          : 17
          : 5
          : 269-278
          aDepartment of Pathology, and b,cNuclear Magnetic Resonance Laboratory, Chicago College of Osteopathic Medicine, Chicago, Ill.; aDepartment of Ophthalmology Center for Sight, Georgetown University, Washington, DC, USA
          265385 Ophthalmic Res 1985;17:269–278
          © 1985 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 13 February 1984
          : 17 October 1984
          Page count
          Pages: 10
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Organophosphates,Ouabain,Crystalline lens,Intact tissue phosphorus-31 nuclear magnetic resonance spectroscopy,Lens pH


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