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      Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

      1 , 2 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 4 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 2 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 29 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ,   50 , 14 , 51 , 52 , 53 , 54 , 55 , 56 , 21 , 57 , 58 , 59 , 55 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 16 , 37 , 71 , 72 , 73 , 56 , 74 , 15 , 75 , 76 , 77 , 2 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 2 , 93 , 94 , 95 , 96 , 2 , 97 , 98 , 99 , 100 , 101 , 98 , 102 , 56 , 85 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 98 , 112 , 113 , 114 , 115 , 85 , 86 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 11 , 42 , 43 , 124 , 2 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 56 , 137 , 82 , 98 , 138 , 139 , 140 , 141 , 142 , 56 , 143 , 144 , 145 , 146 , 147 , 148 , 122 , 2 , 31 , 149 , 150 , 151 , 94 , 152 , 153 , 154 , 155 , 102 , 103 , 2 , 156 , 157 , 158 , 64 , 2 , 159 , 160 , 149 , 161 , 162 , 163 , 85 , 103 , 164 , 130 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 28 , 174 , 175 , 176 , 177 , 20 , 178 , 179 , 31 , 36 , 37 , 77 , 2 , 180 , 181 , 182 , 2 , 183 , 184 , 180 , 185 , 186 , 129 , 98 , 187 , 105 , 188 , 115 , 149 , 56 , 189 , 190 , 98 , 188 , 191 , 175 , 192 , 193 , 194 , 112 , 195 , 196 , 197 , 194 , 198 , 130 , 129 , 98 , 199 , 29 , 200 , 201 , 68 , 70 , 202 , 203 , 204 , 105 , 36 , 37 , 205 , 2 , 97 , 15 , 206 , 105 , 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 , 215 , 12 , 16 , 216 , 217 , 15 , 218 , 219 , 135 , 136 , 220 , 221 , 11 , 222 , 223 , 216 , 68 , 70 , 224 , 225 , 82 , 226 , 227 , 228 , 15 , 56 , 2 , 229 , 146 , 2 , 2 , 180 , 230 , 77 , 231 , 232 , 218 , 207 , 233 , 234 , 232 , 235 , 236 , 237 , 238 , 239 , 149 , 31 , 32 , 240 , 82 , 241 , 98 , 187 , 199 , 242 , 243 , 2 , 244 , 245 , 181 , 241 , 169 , 246 , 247 , 248 , 249 , 250 , 251 , 2 , 252 , 246 , 29 , 153 , 253 , 53 , 254 , 68 , 70 , 255 , 21 , 23 , 256 , 123 , 257 , 149 , 258 , 177 , 77 , 24 , 259 , 236 , 235 , 260 , 261 , 104 , 262 , 263 , 264 , 265 , 241 , 266 , 236 , 267 , 77 , 268 , 168 , 269 , 270 , 254 , 81 , 271 , 272 , 2 , 159 , 273 , 274 , 135 , 275 , 276 , 277 , 198 , 278 , 279 , 280 , 24 , 281 , 112 , 68 , 70 , 282 , 283 , 31 , 32 , 4 , 192 , 14 , 284 , 285 , 286 , 102 , 287 , 288 , 14 , 289 , 77 , 290 , 291 , 2 , 77 , 209 , 292 , 293 , 294 , 295 , 100 , 67 , 296 , 297 , 298 , 299 , 300 , 8 , 168 , 169 , 2 , 159 , 77 , 301 , 302 , 198 , 98 , 187 , 303 , 304 , 305 , 241 , 117 , 2 , 306 , 307 , 308 , 65 , 66 , 300 , 72 , 309 , 310 , 77 , 9 , 311 , 31 , 32 , 312 , 275 , 276 , 313 , 169 , 295 , 130 , 76 , 314 , 315 , 79 , 80 , 316 , 317 , 318 , 319 , 320 , 321 , 44 , 36 , 185 , 186 , 322 , 197 , 263 , 323 , 324 , 325 , 53 , 3 , 2 , 97 , 326 , 24 , 327 , 328 , 329 , 330 , 198 , 4 , 3 , 9 , 98 , 331 , 65 , 66 , 174 , 111 , 332 , 333 , 100 , 334 , 216 , 51 , 335 , 336 , 337 , 180
      European Journal of Immunology
      Wiley
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          Abstract

          These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

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          Most cited references1,452

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              The role of Atg proteins in autophagosome formation.

              Macroautophagy is mediated by a unique organelle, the autophagosome, which encloses a portion of cytoplasm for delivery to the lysosome. Autophagosome formation is dynamically regulated by starvation and other stresses and involves complicated membrane reorganization. Since the discovery of yeast Atg-related proteins, autophagosome formation has been dissected at the molecular level. In this review we describe the molecular mechanism of autophagosome formation with particular focus on the function of Atg proteins and the long-standing discussion regarding the origin of the autophagosome membrane.
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                Author and article information

                Journal
                European Journal of Immunology
                Eur. J. Immunol.
                Wiley
                0014-2980
                1521-4141
                October 21 2019
                October 2019
                October 21 2019
                October 2019
                : 49
                : 10
                : 1457-1973
                Affiliations
                [1 ]Department of Medical and Surgical Sciences for Children and AdultsUniv. of Modena and Reggio Emilia School of Medicine Modena Italy
                [2 ]Deutsches Rheuma‐Forschungszentrum (DRFZ)an Institute of the Leibniz Association Berlin Germany
                [3 ]Department of BiologyNikolaus‐Fiebiger‐Center for Molecular MedicineFriedrich‐Alexander‐University Erlangen‐Nuremberg Erlangen Germany
                [4 ]Institut für ImmunologieChristian‐Albrechts‐Universität zu Kiel Kiel Germany
                [5 ]Institut für TransfusionsmedizinUniversitätsklinik Schleswig‐Holstein Kiel Germany
                [6 ]Mucosal Immunology groupDepartment of Health TechnologyTechnical University of Denmark Kgs. Lyngby Denmark
                [7 ]Immunology SectionLund University Lund Sweden
                [8 ]Departments of Anesthesiology, Pain and Perioperative MedicineBiomedical Data Sciences; and PediatricsStanford University Stanford CA USA
                [9 ]Swiss Institute of Allergy and Asthma Research (SIAF)University of Zurich Davos Switzerland
                [10 ]Université de Paris, Institut de Recherche Saint‐Louis, INSERM U1160, and Gastroenterology DepartmentHôpital Saint‐Louis – APHP Paris France
                [11 ]Institute for Systemic Inflammation ResearchUniversity of Luebeck Luebeck Germany
                [12 ]Laboratory of Translational ImmunologyHumanitas Clinical and Research Center Rozzano Italy
                [13 ]University of Birmingham Birmingham UK
                [14 ]Institut für Medizinische MikrobiologieImmunologie und HygieneTechnische Universität München Munich Germany
                [15 ]Department of Experimental and Clinical MedicineUniversity of Florence Florence Italy
                [16 ]Flow Cytometry CoreHumanitas Clinical and Research Center Milan Italy
                [17 ]Institut für Klinische MolekularbiologieChristian‐Albrechts Universität zu Kiel Germany
                [18 ]Department of Life SciencesUniversity of Siena Siena Italy
                [19 ]Division of Medical SciencesNational Cancer Centre Singapore Singapore
                [20 ]Cancer & Stem Cell BiologyDuke-NUS Medical School Singapore
                [21 ]Dipartimento di Medicina Interna e Specialità MedicheSapienza Università di Roma Rome Italy
                [22 ]Center for Life Nano Science@SapienzaIstituto Italiano di Tecnologia Rome Italy
                [23 ]Istituto Pasteur ‐ Fondazione Cenci Bolognetti Rome Italy
                [24 ]Institute of ImmunologyHannover Medical School Hannover Germany
                [25 ]IRCCS Fondazione Santa Lucia Rome Italy
                [26 ]Division of ImmunologyAllergy and Infectious DiseasesDepartment of DermatologyMedical University of Vienna Vienna Austria
                [27 ]Center for Comparative Medicine & Dept. PathologyMicrobiology & ImmunologyUniversity of California Davis CA USA
                [28 ]Institute for ImmunologyFaculty of MedicineBiomedical Center, LMU Munich, Planegg‐Martinsried Germany
                [29 ]Genomics Core FacilityEuropean Molecular Biology Laboratory (EMBL) Heidelberg Germany
                [30 ]Discipline of PharmacyGraduate School of HealthThe University of Technology Sydney Sydney NSW Australia
                [31 ]Institute of Experimental ImmunologyUniversity of Zurich Zurich Switzerland
                [32 ]Comprehensive Cancer Center Zurich Switzerland
                [33 ]Flow Cytometry Laboratory, Institute of Molecular Toxicology and PharmacologyHelmholtz Zentrum München, German Research Center for Environmental Health München Germany
                [34 ]Department of Biomedical Molecular BiologyCenter for Inflammation ResearchGhent University ‐ VIB Ghent Belgium
                [35 ]Flow Cytometry Core TechnologiesUCD Conway InstituteUniversity College Dublin Dublin Ireland
                [36 ]Department of SurgeryThe University of British Columbia Vancouver Canada
                [37 ]BC Children's Hospital Research Institute Vancouver Canada
                [38 ]Mikrobiologisches Institut ‐ Klinische MikrobiologieImmunologie und Hygiene, Universitätsklinikum Erlangen Erlangen Germany
                [39 ]Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg and Medical Immunology Campus Erlangen Erlangen Germany
                [40 ]Department of Immunology and PathologyMonash University Melbourne Victoria Australia
                [41 ]Neuroimmunology and Flow Cytometry UnitsFondazione Santa Lucia IRCCS Rome Italy
                [42 ]Department of Cell BiologyAlbert Einstein College of Medicine Bronx NY USA
                [43 ]The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research Bronx New York USA
                [44 ]Thermo Fisher Scientific Eugene Oregon USA
                [45 ]Luxembourg Institute of HealthDepartment of Infection and ImmunityExperimental and Molecular Immunology Esch‐sur‐Alzette Luxembourg
                [46 ]Odense University Hospital, Odense Research Center for Anaphylaxis, University of Southern Denmark, Department of Dermatology and Allergy Center Odense Denmark
                [47 ]Luxembourg Centre for Systems Biomedicine (LCSB)University of Luxembourg Belvaux Luxembourg
                [48 ]Department of Medical GeneticsUniversity of British Columbia Vancouver BC Canada
                [49 ]Terry Fox LaboratoryBC Cancer Vancouver BC Canada
                [50 ]University of AucklandSchool of Biological Sciences, Maurice Wilkins Center Auckland New Zealand
                [51 ]German Center for Infection Research (DZIF) Munich Germany
                [52 ]Focus Group ‘‘Clinical Cell Processing and Purification”Institute for Advanced StudyTechnische Universität München Munich Germany
                [53 ]BiophysicsR&D EngineeringMiltenyi Biotec GmbH Bergisch Gladbach Germany
                [54 ]Department of Pediatrics and Shared Resource LaboratoriesUniversity of Rochester Medical Center Rochester NY USA
                [55 ]University of VeronaDepartment of MedicineSection of General Pathology Verona Italy
                [56 ]Department of Microbiology and ImmunologyPeter Doherty Institute for Infection and ImmunityUniversity of Melbourne Parkville Victoria Australia
                [57 ]National Key Laboratory of Medical ImmunologyNankai University Tianjin China
                [58 ]Department of Microbiology and ImmunologyUniversity of Gothenburg Gothenburg Sweden
                [59 ]The FIRC Institute of Molecular Oncology (FOM) Milan Italy
                [60 ]National Institute for HealthMigration and Poverty (INMP) Rome Italy
                [61 ]Macrophage Biology GroupSchool of BiologyUniversity of Barcelona Barcelona Spain
                [62 ]Université Paris DescartesInstitut National de la Santé et de la Recherche Médicale Paris France
                [63 ]Isaac and Laura Perlmutter Cancer CenterNYU‐Langone Medical Center New York USA
                [64 ]Divsion of Medical Oncology and HematologyPrincess Margaret Hospital Toronto Ontario Canada
                [65 ]Department of ImmunobiologySchool of Immunology and Microbial SciencesKing's College London UK
                [66 ]National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health ServiceFoundation Trust and King's College London UK
                [67 ]Department of Vaccinology and Applied MicrobiologyHelmholtz Centre for Infection Research Braunschweig Germany
                [68 ]IRCCS Fondazione Don Carlo Gnocchi Milan Italy
                [69 ]Department of Physiopathology and TransplantsUniversity of Milan Milan Italy
                [70 ]Milan Center for NeuroscienceUniversity of Milano‐Bicocca Milan Italy
                [71 ]Department of MedicineThe University of British Columbia Vancouver Canada
                [72 ]Department of PathologyUniversity of Cambridge Cambridge UK
                [73 ]Department of Respiratory SciencesUniversity of Leicester Leicester UK
                [74 ]National Cytometry PlatformLuxembourg Institute of HealthDepartment of Infection and ImmunityEsch‐sur‐Alzette Luxembourg
                [75 ]de Duve InstituteUniversité catholique de Louvain Brussels Belgium
                [76 ]Unit LymphopoiesisDepartment of ImmunologyInstitut Pasteur Paris France
                [77 ]Division of Molecular ImmunologyNikolaus‐Fiebiger‐CenterDept. of Internal Medicine IIIUniversity of Erlangen‐Nuremberg Erlangen Germany
                [78 ]Clinical Research UnitBerlin Institute of Health (BIH)Charite Universitätsmedizin Berlin Berlin Germany
                [79 ]Infection and Immunity Program and Department of Biochemistry and Molecular BiologyBiomedicine Discovery InstituteMonash University Clayton Victoria Australia
                [80 ]Australian Research Council Centre of Excellence in Advanced Molecular ImagingMonash University Clayton Victoria Australia
                [81 ]Flow Cytometry Scientific Technology PlatformThe Francis Crick Institute London UK
                [82 ]Department of SurgeryMedicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia Modena Italy
                [83 ]IRCCS Istituto Giannina Gaslini Genova Italy
                [84 ]Novo Nordisk Foundation Center for Stem Cell Biology – DanStemUniversity of Copenhagen Copenhagen Denmark
                [85 ]Regensburg Center for Interventional Immunology (RCI) Regensburg Germany
                [86 ]Chair for ImmunologyUniversity Regensburg Germany
                [87 ]Department of Medical Biotechnologies and Translational MedicineUniversity of Milan Milan Italy
                [88 ]Division of ImmunologyTransplantation and Infectious DiseasesSan Raffaele Scientific Institute Milan Italy
                [89 ]Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology Istanbul Turkey
                [90 ]Sony Europe Ltd Weybridge UK
                [91 ]Innate Immunty UnitDepartment of ImmunologyInstitut Pasteur Paris France
                [92 ]Institut Pasteur Inserm U1223 Paris France
                [93 ]Charité ‐ Universitätsmedizin BerlinLaboratory of Innate ImmunityDepartment of MicrobiologyInfectious Diseases and Immunology Berlin Germany
                [94 ]Berlin Institute of Health (BIH) Berlin Germany
                [95 ]University of PalermoCentral Laboratory of Advanced Diagnosis and Biomedical ResearchDepartment of BiomedicineNeurosciences and Advanced Diagnostics Palermo Italy
                [96 ]Flow Cytometry Core FacilityInstitute of Experimental ImmunologyUniversity of Bonn Bonn Germany
                [97 ]Dept. Medicine/Rheumatology and Clinical ImmunologyCharité Universitätsmedizin Berlin Germany
                [98 ]Singapore Immunology Network (SIgN)A*STAR (Agency for Science, Technology and Research) Biopolis Singapore
                [99 ]Department of DermatologyLaboratory of Dendritic Cell BiologyFriedrich‐Alexander Universität Erlangen‐Nürnberg (FAU)University Hospital Erlangen Erlangen Germany
                [100 ]Kennedy Institute of RheumatologyUniversity of Oxford Oxford UK
                [101 ]Program in Emerging Infectious DiseaseDuke‐NUS Medical School Singapore
                [102 ]Institute of ImmunologyCentre for Infection MedicineDepartment of Veterinary MedicineFreie Universität Berlin Germany
                [103 ]Department of Internal Medicine IIIUniversity Hospital Regensburg Germany
                [104 ]Charité – Universitätsmedizin Berlincorporate member of Freie Universität Berlin, Humboldt‐Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology Germany
                [105 ]Department of ImmunologyUniversity of Toronto Toronto ON Canada
                [106 ]Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI‐STEM gGmbH) Heidelberg Germany
                [107 ]University of Barcelona, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences Barcelona Spain
                [108 ]Theodor Kocher InstituteUniversity of Bern Bern Switzerland
                [109 ]Department of ImmunologyUniversity L. Eotvos Budapest Hungary
                [110 ]Leibniz Research Institute for Environmental Medicine Düsseldorf Germany
                [111 ]Department of ParasitologyLeiden University Medical Center Leiden The Netherlands
                [112 ]Institute of Transplant ImmunologyHannover Medical SchoolMHH Hannover Germany
                [113 ]Division of OncologyWashington University School of Medicine St. Louis MO USA
                [114 ]Laboratory of CytomicsJoint Research Unit CIPF‐UVEGPrincipe Felipe Research Center Valencia Spain
                [115 ]Experimental Medicine DivisionNuffield Department of MedicineUniversity of Oxford Oxford UK
                [116 ]The Flow Cytometry Core FacilityFaculty of Medical SciencesNewcastle University Newcastle upon Tyne UK
                [117 ]MDQuest Ltd. Szeged Hungary
                [118 ]Institut Necker‐Enfants MaladesUniversité Paris Descartes Sorbonne Paris Cité, Faculté de Médecine, AP‐HP, Hôpital Necker Enfants Malades INSERM U1151‐CNRS UMR 8253 Paris France
                [119 ]Department of Medicine IMedical Center ‐ University of FreiburgFaculty of MedicineUniversity of Freiburg Freiburg Germany
                [120 ]Universitaetsklinikum FreiburgLighthouse Core FacilityZentrum für Translationale Zellforschung, Klinik für Innere Medizin I Freiburg Germany
                [121 ]Immunology and EnvironmentLIMES InstituteUniversity of Bonn Bonn Germany
                [122 ]Owl Biomedical Inc. Santa Barbara USA
                [123 ]John van Geest Cancer Research CentreNottingham Trent University Nottingham UK
                [124 ]Department of MedicineAlbert Einstein College of Medicine Bronx NY USA
                [125 ]Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt‐Universität zu Berlin, and Berlin Institute of HealthDepartment of Dermatology, Venereology and Allergology
                [126 ]Nanobiophotonics DepartmentLeibniz Institute of Photonic Technology (IPHT) Jena Germany
                [127 ]School of Plant Sciences and Bio5 InstituteUniversity of Arizona Tucson USA
                [128 ]Honorary Dean of Life SciencesHenan University Kaifeng China
                [129 ]Division of Molecular Oncology and Immunologythe Netherlands Cancer Institute Amsterdam The Netherlands
                [130 ]Institute of Experimental ImmunologyUniversity of Bonn Germany
                [131 ]Stanford Department of AnesthesiologyPerioperative and Pain MedicineStanford University School of Medicine CA USA
                [132 ]Fundação Oswaldo Cruz ‐ MinasLaboratory of Immunopatology Belo Horizonte MG Brazil
                [133 ]Department of MecicineUniversity of Massachusetts Medical School Worcester MA USA
                [134 ]INGM ‐ Fondazione Istituto Nazionale di Genetica Molecolare “Ronmeo ed Enrica Invernizzi” Milan Italy
                [135 ]Institute of ImmunologyDepartment of PathobiologyUniversity of Veterinary Medicine Vienna Austria
                [136 ]Christian Doppler Laboratory for Optimized Prediction of Vaccination Success in PigsInstitute of ImmunologyDepartment of PathobiologyUniversity of Veterinary Medicine Vienna Austria
                [137 ]Department of DermatologyVenereology and Allergology, Charité ‐ Universitätsmedizin Berlin Berlin Germany
                [138 ]Translational Immunology InstituteSingHealth Duke‐NUS Academic Medical Centre Singapore
                [139 ]Shanghai Institute of ImmunologyDepartment of Immunology and MicrobiologyShanghai Jiao Tong University School of Medicine Shanghai China
                [140 ]Department of BioengineeringUniversity of California Los Angeles California USA
                [141 ]Department of ChemistryUniversity of Tokyo Tokyo Japan
                [142 ]Institute of Technological SciencesWuhan University Wuhan China
                [143 ]Institut für GenetikUniversität zu Köln Köln Germany
                [144 ]Alicante Institute for Health and Biomedical Research (ISABIAL) Alicante Spain
                [145 ]Networked Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd) Madrid Spain
                [146 ]Institute of Infection Immunity and InflammationCollege of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre Glasgow UK
                [147 ]Fondazione IRCCS Ca’ Granda, Ospedale Maggiore PoliclinicoUniversity of Milan
                [148 ]Cancer Immunology Research, Genentech South San Francisco CA USA
                [149 ]Friedrich‐Alexander‐University Erlangen‐Nürnberg (FAU)Department of Medicine 3Rheumatology and ImmunologyUniversitätsklinikum Erlangen Erlangen
                [150 ]Department of Internal Medicine and PediatricsFaculty of Medicine and Health Sciences Zwijnaarde Belgium
                [151 ]German Cancer Research Center (DKFZ) Heidelberg Germany
                [152 ]German Cancer Consortium (DKTK), partner site Berlin Berlin Germany
                [153 ]Department of Hematology, Oncology, and Tumor ImmunologyCharité – Universitätsmedizin Berlin, Campus Virchow Klinikum Berlin Germany
                [154 ]Department of Medicine and Center for Molecular Medicine at Karolinska University Hospital Solna Sweden
                [155 ]Murdoch Children's Research Institute Parkville Victoria Australia
                [156 ]Charité – Universitätsmedizin Berlincorporate member of Freie Universität Berlin, Humboldt‐Universität zu Berlin
                [157 ]Department of Rheumatology and Clinical ImmunologyBerlin Institute of Health Berlin Germany
                [158 ]Flow CytometryHouston Methodist Hospital Research Institute Houston TX USA
                [159 ]Charité ‐ Universitätsmedizin BerlinMedical Department IDivision of GastroenterologyInfectiology and Rheumatology Berlin Germany
                [160 ]Cytometry ServiceIncliva Foundation. Clinic Hospital and Faculty of MedicineUniversity of Valencia Valencia Spain
                [161 ]Immunobiology Laboratory, Department of BiomedicineUniversity and University Hospital Basel Basel Switzerland
                [162 ]Cambridge Institute of Therapeutic Immunology & Infectious DiseaseJeffrey Cheah Biomedical CentreUniversity of Cambridge Cambridge UK
                [163 ]German Cancer Research Center (DKFZ)Division of Theoretical Systems Biology Heidelberg Germany
                [164 ]Center for ImmunologyUniversity of Minnesota Minneapolis MN USA
                [165 ]Leiden Computational Biology CenterLeiden University Medical Center Leiden The Netherlands
                [166 ]Computer Graphics and VisualizationDepartment of Intelligent SystemsTU Delft Delft The Netherlands
                [167 ]Karolinska Institutet Biomedicum Solna Sweden
                [168 ]Department of Experimental ImmunologyAmsterdam Infection and Immunity InstituteAmsterdam UMCUniversity of Amsterdam Amsterdam The Netherlands
                [169 ]Department of HematopoiesisSanquin Research and Landsteiner LaboratoryAmsterdam UMCUniversity of Amsterdam Amsterdam The Netherlands
                [170 ]Department of Chemical & Materials EngineeringNew Mexico State University Las Cruces NM USA
                [171 ]Rheumatologie/Klinische Immunologie, Klinik für Innere Medizin I und Exzellenzzentrum EntzündungsmedizinUniversitätsklinikum Schleswig‐Holstein Kiel Germany
                [172 ]Department of Immunology & National Key Laboratory of Medical Molecular BiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences (CAMS) & Peking Union Medical College Beijing China
                [173 ]Institute for Advanced Study (IAS)Shenzhen University Shenzhen China
                [174 ]Experimental ImmunologyHelmholtz Centre for Infection Research Braunschweig Germany
                [175 ]Department of HematologyOncology and RheumatologyUniversity Heidelberg Heidelberg Germany
                [176 ]Department of PathobiologySchool of Veterinary Medicine, University of Pennsylvania Philadelphia PA USA
                [177 ]Department of HematologySingapore General Hospital Singapore
                [178 ]Executive OfficesNational Cancer Centre Singapore Singapore
                [179 ]Department of Diagnostic MedicineClinical and Public Health, Univ. of Modena and Reggio Emilia Modena Italy
                [180 ]Max Planck Institute for Infection Biology Berlin Germany
                [181 ]Laboratory of CytomicsJoint Research Unit CIPF‐UVEGDepartment of Biochemistry and Molecular BiologyUniversity of Valencia Valencia Spain
                [182 ]Department of Histology and Embryology/Center for ProteomicsFaculty of Medicine, University of Rijeka Rijeka Croatia
                [183 ]Department of Paediatric Haematology and OncologySecond Faculty of MedicineCharles University Prague Czech Republic
                [184 ]Jena University HospitalInstitute of Immunology Jena Germany
                [185 ]Department of Rheumatology and Clinical ImmunologyMedical Center ‐ University of FreiburgFaculty of Medicine, University of Freiburg Freiburg Germany
                [186 ]Center for Chronic ImmunodeficiencyMedical Center ‐ University of FreiburgFaculty of MedicineUniversity of Freiburg Freiburg Germany
                [187 ]Department of Microbiology and ImmunologyYong Loo Lin School of MedicineNational University of Singapore Singapore
                [188 ]Institute of Molecular Health SciencesETH Zurich Zürich Switzerland
                [189 ]Department of NeuropathologyUniversitätsklinikum Erlangen Germany
                [190 ]John W Deming Endowed Chair in Internal MedicineCenter for Translational Research in Infection and Inflammation Tulane School of Medicine New Orleans LA USA
                [191 ]Department of NeurologyTechnical University of Munich Munich Germany
                [192 ]Department of RheumatologyLeiden University Medical Center Leiden The Netherlands
                [193 ]Division of Cell BiologyHistology & EmbryologyGottfried Schatz Research CenterMedical University of Graz Graz Austria
                [194 ]Institute for Molecular MedicineGoethe University Frankfurt Frankfurt am Main Germany
                [195 ]FACS & Imaging Core FacilityMax Planck Institute for Biology of Ageing Cologne Germany
                [196 ]Flow & Mass Cytometry Core FacilityCharité ‐ Universitätsmedizin Berlin and Berlin Institute of Health Berlin Germany
                [197 ]BCRT Flow Cytometry LabBerlin‐Brandenburg Center for Regenerative TherapiesCharité ‐ Universitätsmedizin Berlin
                [198 ]WPI Immunology Frontier Research CenterOsaka University Osaka Japan
                [199 ]School of Biological SciencesNanyang Technological University Singapore
                [200 ]INSERM U932PSL UniversityInstitut Curie Paris France
                [201 ]Department of Medicine and Aging SciencesCentre on Aging Sciences and Translational Medicine (Ce.S.I.‐Me.T.), University “G. d'Annunzio” of Chieti‐Pescara Chieti Italy
                [202 ]Institut Cochin, CNRS8104, INSERM1016Department of Endocrinology, Metabolism and DiabetesUniversité de Paris Paris France
                [203 ]Section of ImmunologyVetsuisse FacultyUniversity of Zurich Zurich Switzerland
                [204 ]The Human Immune Monitoring Center (HIMC)Institute for ImmunityTransplantation and InfectionStanford University School of Medicine CA USA
                [205 ]School of Biomedical EngineeringThe University of British Columbia Vancouver Canada
                [206 ]Caprion BiosciencesImmuneCarta Montréal Quebec Canada
                [207 ]Center for Infectious MedicineDepartment of Medicine HuddingeANA Futura, Karolinska Institutet Stockholm Sweden
                [208 ]Inst. f. Med. Mikrobiology and Hospital HygieneUniversity of Marburg Germany
                [209 ]King's College London“Peter Gorer” Department of Immunobiology London UK
                [210 ]Beckman Coulter, Inc Miami FL USA
                [211 ]IMIM(Hospital de Mar Medical Research Institute)University Pompeu Fabra Barcelona Spain
                [212 ]Department of Microbial Infection and ImmunityOhio State University Columbus OH USA
                [213 ]Department of RheumatologyErasmus MC, University Medical Center Rotterdam Rotterdam The Netherlands
                [214 ]Centre d'Immunophénomique ‐ CIPHE (PHENOMIN)Aix Marseille Université (UMS3367), Inserm (US012), CNRS (UMS3367) Marseille France
                [215 ]Institute of ImmunobiologyKantonsspital St.Gallen St. Gallen Switzerland
                [216 ]Department of Virus ImmunologyHeinrich Pette InstituteLeibniz Institute for Experimental Virology Hamburg Germany
                [217 ]Institute for ImmunityTransplantation, and Infection, Stanford University School of Medicine Stanford CA USA
                [218 ]Flow and Image Cytometry Shared ResourceRoswell Park Comprehensive Cancer Center Buffalo NY USA
                [219 ]Fred Hutchinson Cancer Research CenterVaccine and Infectious Disease Division Seattle WA USA
                [220 ]Istituto Clinico Humanitas IRCCS and Humanitas University Pieve Emanuele Milan Italy
                [221 ]William Harvey Research InstituteQueen Mary University London United Kingdom
                [222 ]Department of ImmunologyRady Faculty of Health Sciences, University of Manitoba Winnipeg MB Canada
                [223 ]Cytomics Technological ServicePrincipe Felipe Research Center Valencia Spain
                [224 ]National Institute of GeriatricsRheumatology and RehabilitationDepartment of Pathophysiology and Immunology Warsaw Poland
                [225 ]Treg Cell Lab, Dipartimento di Medicina Molecolare e Biotecologie MedicheUniversità di Napoli Federico II and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS‐CNR) Napoli Italy
                [226 ]Lab of Clinical and Experimental ImmunologyHumanitas Clinical and Research Center Rozzano Milan Italy
                [227 ]Cell Clearance in Health and Disease LabVIB Center for Inflammation Research Ghent Belgium
                [228 ]Department of Biomedical Molecular BiologyGhent University Ghent Belgium
                [229 ]Ramaciotti Facility for Human Systems Biology, and Discipline of PathologyThe University of Sydney Camperdown Australia
                [230 ]Clinical Trial Center LeipzigUniversity Leipzig Leipzig Germany
                [231 ]Interdepartmental Immunobiology CenterDept. of Microbiology‐ImmunologyNorthwestern Univ. Medical School Chicago IL USA
                [232 ]Trinity College Dublin, School of Biochemistry and ImmunologyTrinity Biomedical Sciences Institute Dublin Ireland
                [233 ]Department of Clinical and Experimental MedineLinköping University Linköping Sweden
                [234 ]Abramson Cancer Center Flow Cytometry and Cell Sorting Shared ResourcePerelman School of Medicine at the University of Pennsylvania Philadelphia PA USA
                [235 ]Department of ImmunologyIRCCS Bambino Gesu Children's Hospital Rome Italy
                [236 ]David H. Smith Center for Vaccine Biology and ImmunologyUniversity of Rochester Medical Center Rochester NY USA
                [237 ]Centre for Environmental Research ‐ UFZDepartment Environmental Microbiology Leipzig Germany
                [238 ]Institute for Innovative Radiotherapy (iRT)Experimental Immune Biology, Helmholtz Zentrum München Neuherberg Germany
                [239 ]Radiation Immuno‐Oncology GroupCenter for Translational Cancer Research Technische Universität München (TranslaTUM)Klinikum rechts der Isar Munich Germany
                [240 ]Department of ImmunologyGraduate School of MedicineChiba University Chiba city Chiba Japan
                [241 ]Institute of Experimental Immunology and HepatologyUniversity Medical Center Hamburg‐Eppendorf Hamburg Germany
                [242 ]Discipline of DermatologyUniversity of Sydney Sydney New South Wales Australia
                [243 ]State Key Laboratory of Experimental HematologyInstitute of HematologyChinese Academy of Medical Sciences & Peking Union Medical College Tianjin China
                [244 ]Barts and The London School of Medicine and DentistryQueen Mary University of London UK
                [245 ]Department of Pathology and Rogel Cancer Centerthe University of Michigan Ann Arbor Michigan USA
                [246 ]Flow Cytometry Core FacilityEuropean Molecular Biology Laboratory (EMBL) Heidelberg Germany
                [247 ]Department of MedicineCancer Research Centre (IBMCC‐CSIC/USAL)Cytometry ServiceUniversity of SalamancaCIBERONC and Institute for Biomedical Research of Salamanca (IBSAL) Salamanca Spain
                [248 ]Burnet InstituteAMREP Flow Cytometry Core Facility Melbourne Victoria Australia
                [249 ]Inflammation and OncologyResearch, Amgen Inc South San Francisco USA
                [250 ]ETH ZurichInstitute of Microbiology Zurich Switzerland
                [251 ]Department of Transfusion MedicineInstitute of Immunology and Transfusion MedicineUniversity Medicine Greifswald Greifswald Germany
                [252 ]Center of Excellence for Flow CytometryDepartment of Research and DevelopmentFaculty of Medicine Siriraj Hospital, Mahidol University Bangkok Thailand
                [253 ]Institute of Biomedicine and Translational MedicineUniversity of Tartu Tartu Estonia
                [254 ]Functional Cytomics GroupJosep Carreras Leukaemia Research InstituteCampus ICO‐Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB Badalona Spain
                [255 ]Institute of ImmunologyCenter for PathophysiologyInfectiology and Immunology, Medical University of Vienna Vienna Austria
                [256 ]Department of Life SciencesUniversity of Modena and Reggio Emilia Modena Italy
                [257 ]Chromocyte LimitedElectric Works Sheffield UK
                [258 ]Department for Internal Medicine 3Institute for Rheumatology and ImmunologyAG Munoz, Universitätsklinikum Erlangen Erlangen Germany
                [259 ]Department of ImmunologyUniversity of Udine Udine Italy
                [260 ]School of Biomedical and Health SciencesRMIT University Bundoora Victoria Australia
                [261 ]Department of Biochemistry and Molecular BiologyMonash University Clayton Victoria Australia
                [262 ]Department of Rheumatology and Clinical ImmunologyUniversity Medical Center UtrechtUtrecht University Utrecht The Netherlands
                [263 ]Regeneration in HematopoiesisLeibniz‐Institute on AgingFritz‐Lipmann‐Institute (FLI) Jena Germany
                [264 ]Preparative Flow Cytometry, Max‐Delbrück‐Centrum für Molekulare Medizin Berlin Germany
                [265 ]Bindley Biosciences CenterPurdue University West Lafayette IN USA
                [266 ]Department of Internal MedicineGroene Hart Hospital Gouda The Netherlands
                [267 ]Desatoya LLC Reno NV USA
                [268 ]Immunobiology LaboratoryThe Francis Crick Institute London UK
                [269 ]Renal Transplant UnitDivision of Internal MedicineAcademic Medical CentreAmsterdam UMCUniversity of Amsterdam Amsterdam The Netherlands
                [270 ]Core Facility Flow CytometryBiomedical Center, Ludwig‐Maximilians‐University Munich Germany
                [271 ]Department of Medical Microbiology and ImmunologyUniversity of Alberta Alberta Canada
                [272 ]Purdue University Cytometry LaboratoriesPurdue University West Lafayette IN USA
                [273 ]Cell Biology UnitIRCCS Ospedale Policlinico San Martino Genova Italy
                [274 ]Institut für Klinische Chemie und PathobiochemieFakultät für MedizinTechnische Universität München München Germany
                [275 ]Data Mining and Modeling for BiomedicineVIB‐UGent Center for Inflammation Research Ghent Belgium
                [276 ]Department of Applied Mathematics, Computer Science and StatisticsGhent University Ghent Belgium
                [277 ]RIKEN Center for Integrative Medical Sciences Yokohama Japan
                [278 ]Flow Cytometry Facility, Ludwig Cancer Institute, Faculty of Medicine and BiologyUniversity of Lausanne Epalinges Switzerland
                [279 ]Heidelberg UniversityInstitute of ImmunologySection of Molecular Immunology Heidelberg Germany
                [280 ]Translational Immunology LaboratoryNIHR BRCUniversity of OxfordKennedy Institute of Rheumatology Oxford UK
                [281 ]Department of Molecular MedicineSapienza University of RomeIRCCS Neuromed Pozzilli Italy
                [282 ]Centre de Recherches des CordeliersEquipe Cancer et Immunité anti‐tumorale Paris France
                [283 ]Charité – Universitätsmedizin Berlin, and Berlin Institute of HealthInstitute of Medical Immunology Berlin Germany
                [284 ]Clinic for Orthopedics and Trauma SurgeryUniversity Hospital Bonn Bonn Germany
                [285 ]R&D ReagentsMiltenyi Biotec GmbH Bergisch Gladbach Germany
                [286 ]Quantitative Systems BiologyLife & Medical Sciences InstituteUniversity of Bonn Bonn Germany
                [287 ]Internal Medicine I, University Hospital Regensburg Germany
                [288 ]Flow Cytometry Core FacilityGerman Cancer Research Centre (DKFZ) Heidelberg Germany
                [289 ]Genome Biology UnitEuropean Molecular Biology Laboratory (EMBL) Heidelberg Germany
                [290 ]Institute of Molecular and Clinical ImmunologyOtto‐von‐Guericke University Magdeburg Germany
                [291 ]University of CambridgeCambridge Institute for Medical Research Cambridge UK
                [292 ]Institut PasteurCellular Lymphocytes BiologyImmunology Departement Paris France
                [293 ]TRI Flow Cytometry Suite (TRI.fcs)Translational Research Institute Wooloongabba QLD Australia
                [294 ]AsedaSciences West Lafayette IN USA
                [295 ]Instituto de Medicina Molecular João Lobo AntunesFaculdade de MedicinaUniversidade de Lisboa Portugal
                [296 ]Dept. Molecular Translational MedicineUniversity of Brescia Brescia Italy
                [297 ]Department of OncologyUniversity of Lausanne and CHUV Epalinges Switzerland
                [298 ]BD Life Sciences Ashland OR USA
                [299 ]Lundberg Laboratory for CancerDepartment of PathologySahlgrenska Academy at University of Gothenburg Gothenburg Sweden
                [300 ]BD Life Sciences San Diego CA USA
                [301 ]Institute for Hygiene and Applied ImmunologyCenter for PathophysiologyInfectiology and ImmunologyMedical University of Vienna Vienna Austria
                [302 ]National Institutes of Health Bethesda MD USA
                [303 ]Departement for Therapy ValidationFraunhofer Institute for Cell Therapy and Immunology IZI Leipzig Germany
                [304 ]Institute for Medical InformaticsStatistics and Epidemiology (IMISE)University of Leipzig Leipzig Germany
                [305 ]Department of Precision InstrumentsTsinghua University Beijing China
                [306 ]BioGenes GmbH Berlin Germany
                [307 ]Novartis Biologics CenterMechanistic Immunology UnitNovartis Institute for Biomedical ResearchNIBR Basel Switzerland
                [308 ]Department of Cellular and Molecular PhysiologyPenn State University College of Medicine PA United States
                [309 ]Institute of BioinnovationBSRC Alexander Fleming Vari Greece
                [310 ]Department of Biochemistry, Institute of ChemistryUniversity of São Paulo São Paulo SP Brazil
                [311 ]Christine Kühne Center for Allergy Research and Education (CK‐CARE) Davos Switzerland
                [312 ]Vesicle Observation CenterBiomedical Engineering & PhysicsLaboratory Experimental Clinical ChemistryAmsterdam University Medical Centers Location AMC The Netherlands
                [313 ]VIB Flow CoreVIB Center for Inflammation Research Ghent Belgium
                [314 ]Department of Infection BiologyUniversity Hospital ErlangenFriedrich‐Alexander University Erlangen‐Nuremberg (FAU) Erlangen Germany
                [315 ]BIH Center for Regenerative Therapies (BCRT) Charité Universitätsmedizin Berlin and Berlin Institute of HealthCore Unit ImmunoCheck
                [316 ]A·P·E‐Applied Physics & Electronics Berlin Germany
                [317 ]Institute for Molecular MedicineUniversity Medical Center of the Johannes Gutenberg University of Mainz Mainz Germany
                [318 ]Flow Cytometry FacilityBabraham Institute Cambridge UK
                [319 ]Roswell Park Comprehensive Cancer CenterElm and Carlton Streets Buffalo NY USA
                [320 ]Dept of HematopathologyThe University of Texas MD Anderson Cancer Center Houston TX USA
                [321 ]The Scientific Platformsthe Westmead Institute for Medical Researchthe Westmead Research Hub Westmead New South Wales Australia
                [322 ]Flow Cytometry Core FacilityBlizard InstituteQueen Mary London University London UK
                [323 ]Faculty of Biological SciencesFriedrich Schiller University Jena Jena Germany
                [324 ]Medinfomatics Ltd. West Drayton Middlesex UK
                [325 ]Department for ImmunologyLeibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo) Dortmund Germany
                [326 ]Institute for Cellular & Molecular ImmunologyUniversity Medical Center Göttingen Göttingen Germany
                [327 ]Department of Infectious DiseaseImperial College London UK
                [328 ]Wellcome Centre for Infectious Diseases Research in Africa and Department of MedicineInstitute of Infectious Disease and Molecular MedicineUniversity of Cape Town, Republic of South Africa
                [329 ]Tuberculosis LaboratoryThe Francis Crick Institute London UK
                [330 ]Cooperation Unit for Experimental and Translational Cancer ImmunologyInstitute of Immunology (Charité ‐ Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ) Berlin Germany
                [331 ]University BonnMedical Faculty Bonn Germany
                [332 ]BD Biosciences San Jose CA USA
                [333 ]School of Computing ScienceSimon Fraser University Burnaby Canada
                [334 ]Department of Rheumatology and ImmunologyWest China Hospital, Sichuan University Chengdu Sichuan China
                [335 ]Institute of VirologyTechnical University of Munich Munich Germany
                [336 ]TranslaTUMTechnical University of Munich Munich Germany
                [337 ]Maurice Müller Laboratories (Department of Biomedical Research)Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern Bern Switzerland
                Article
                10.1002/eji.201970107
                7350392
                31633216
                eb5154ad-6864-40a3-abd0-f168d1cfb7cf
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