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      Genetic diversity of the Common Black Hawk (Buteogallus anthracinus) population in Los Tuxtlas, Mexico, based on microsatellite markers Translated title: Diversidad genética de la población de la aguililla negra menor (Buteogallus anthracinus) en Los Tuxtlas, México, basada en marcadores microsatelitales

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          Abstract

          Abstract The Common Black Hawk (Buteogallus anthracinus) is a raptor associated with wetlands. Many of its populations are in decline as a consequence of habitat loss and degradation. However, there are no published studies on the genetic variation of their populations. We characterize for the first time the genetic variation of a population of B. anthracinus in southern Veracruz, Mexico. We used feathers from 19 individuals to extract DNA and amplify and genotype 9 microsatellite loci. Samples were collected from nests (n = 4), at feeding sites (n = 5), and directly from chicks (n = 2), young individuals (n = 7), and 1 adult. Six out of 9 microsatellites were polymorphic and 3 monomorphic for this population. The highest number of alleles (n = 7) was observed at the BswD107w, with an average of 4.2 alleles per polymorphic locus. Mean observed and expected heterozygosity were 0.41 and 0.53, respectively. These values are on the lower end of those found for other birds of prey, but there is no evidence of high levels of inbreeding in the population. The polymorphic microsatellite loci analyzed in this study provide a useful tool to continue characterizing the genetic variation of B. anthracinus populations and evaluate possible inter-population differences.

          Translated abstract

          Resumen El Aguililla negra (Buteogallus anthracinus) es una rapaz asociada con humedales. Muchas de sus poblaciones están en declive por pérdida y transformación del hábitat. Sin embargo, no existen estudios que analicen los niveles de su variación genética. Reportamos la primera caracterización de la variabilidad genética en una población de B. anthracinus de Veracruz, México, mediante el uso de marcadores moleculares. Utilizamos plumas de 19 individuos para extraer ADN, amplificar y genotipificar 9 loci de microsatélites. Las plumas fueron recolectadas en nidos (n = 4), en sitios de alimentación (n = 5), directamente de los pollos (n = 2), de individuos inmaduros (n = 7) y de 1 individuo adulto. Seis de los 9 microsatélites resultaron polimórficos y 3 monomórficos para la población. El número más alto de alelos (n = 7) se observó en el marcador BswD107w, con un promedio de 4.2 alelos por locus polimórfico. Los promedios de heterocigosidad observada y esperada fueron 0.41 y 0.53, respectivamente. Aunque estos valores son relativamente bajos comparados con otras especies de rapaces, no se encontró evidencia de altos niveles de endogamia. Los microsatélites polimórficos analizados proveen una herramienta para continuar caracterizando genéticamente poblaciones de B. anthracinus y analizar posibles diferencias interpoblaciones.

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                Author and article information

                Journal
                rmbiodiv
                Revista mexicana de biodiversidad
                Rev. Mex. Biodiv.
                Instituto de Biología (México, DF, Mexico )
                1870-3453
                2007-8706
                2020
                : 91
                : e902774
                Affiliations
                [2] orgnameUniversity of Michigan orgdiv1Department of Ecology and Evolutionary Biology United States
                [1] Xalapa orgnameUniversidad Veracruzana orgdiv1Instituto de Neuroetología orgdiv2Laboratorio Biología de la Conducta Mexico
                Article
                S1870-34532020000100211 S1870-3453(20)09100000211
                10.22201/ib.20078706e.2020.91.2774
                eb5911c3-6579-4e44-a396-58e6a78cffc7

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 03 October 2018
                : 02 October 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 56, Pages: 0
                Product

                SciELO Mexico

                Categories
                Conservation

                Veracruz,DNA,Genética de la población,Rapaz,Population genetics,ADN,Manglar,Mangrove,Bird of prey

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