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      A TICT based NIR-fluorescent probe for human serum albumin: a pre-clinical diagnosis in blood serum

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          Abstract

          A TICT based NIR-fluorescent probe 3has been designed and synthesized which selectively detects HSA with fluorescence enhancement in blood plasma with a detection limit of 11 nM among the various proteins, nucleotides and thiols tested.

          Abstract

          A TICT based NIR-fluorescent probe 3has been designed and synthesized, which selectively detects HSA with fluorescence enhancement in blood plasma with a detection limit of 11 nM among the various proteins, nucleotides and thiols tested.

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          Most cited references26

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          Creating new fluorescent probes for cell biology.

          Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.
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            Structure of Serum Albumin

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              Specific light-up bioprobes based on AIEgen conjugates.

              Driven by the high demand for sensitive and specific tools for optical sensing and imaging, bioprobes with various working mechanisms and advanced functionalities are flourishing at an incredible speed. Conventional fluorescent probes suffer from the notorious effect of aggregation-caused quenching that imposes limitation on their labelling efficiency or concentration to achieve desired sensitivity. The recently emerged fluorogens with an aggregation-induced emission (AIE) feature offer a timely remedy to tackle the challenge. Utilizing the unique properties of AIE fluorogens (AIEgens), specific light-up probes have been constructed through functionalization with recognition elements, showing advantages such as low background interference, a high signal to noise ratio and superior photostability with activatable therapeutic effects. In this tutorial review, we summarize the recent progress in the development of specific AIEgen-based light-up bioprobes. Through illustration of their operation mechanisms and application examples, we hope to provide guidelines for the design of more advanced AIE sensing and imaging platforms with high selectivity, great sensitivity and wide adaptability to a broad range of biomedical applications.
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                Author and article information

                Journal
                CHCOFS
                Chemical Communications
                Chem. Commun.
                Royal Society of Chemistry (RSC)
                1359-7345
                1364-548X
                2016
                2016
                : 52
                : 6
                : 1182-1185
                Affiliations
                [1 ]Department of Chemistry
                [2 ]UGC Sponsored Centre for Advanced Studies-1
                [3 ]Guru Nanak Dev University
                [4 ]Amritsar
                [5 ]India
                Article
                10.1039/C5CC08217J
                eb6687ac-d834-47e4-af87-b44939bdf11f
                © 2016
                History

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