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      Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications

      , , , , , ,
      Yeast
      Wiley

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          Abstract

          A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes.

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          Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.

          A set of GAL2+ yeast strains that are isogenic to strain S288C have been constructed. They contain non-reverting mutations in genes commonly used for selection for recombinant plasmids. Strains from this collection are being used for the European Union Yeast Genome Sequencing Programme. Representative strains from this collection have been deposited with the ATCC.
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            New heterologous modules for classical or PCR-based gene disruptions inSaccharomyces cerevisiae

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              A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

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                Author and article information

                Journal
                Yeast
                Yeast
                Wiley
                0749503X
                10970061
                January 30 1998
                January 30 1998
                : 14
                : 2
                : 115-132
                Article
                10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2
                9483801
                ebadfe83-df9e-4776-8f56-1d0cb265a9e1
                © 1998

                http://doi.wiley.com/10.1002/tdm_license_1.1

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