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      Development of 18 novel microsatellite primers for Begonia fimbristipula (Begoniaceae), an endangered medicinal plant in China 1

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          Abstract

          Premise of the study:

          Begonia fimbristipula (Begoniaceae) is a medicinal herb distributed in the Chinese provinces of Fujian, Guangdong, Guangxi, Hainan, Hunan, Jiangxi, and Zhejiang, and it is on the verge of extinction due to habitat destruction and deterioration of its ecosystem. Here we developed a set of highly polymorphic microsatellite markers for population genetic and conservation studies of this endangered medicinal plant.

          Methods and Results:

          Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 18 polymorphic microsatellite markers were identified within 48 individuals from two geographic locations. The observed and expected heterozygosities ranged from 0.208 to 1.000 and from 0.291 to 0.812, respectively. These microsatellite markers were cross-amplified in five related Begonia species, and six loci were successfully amplified in all species.

          Conclusions:

          These 18 markers will be useful for better conservation and utilization of wild resources of B. fimbristipula and other Begonia species in the future.

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          Most cited references2

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          Strategies for microsatellite isolation: a review.

          In the last few years microsatellites have become one of the most popular molecular markers used with applications in many different fields. High polymorphism and the relative ease of scoring represent the two major features that make microsatellites of large interest for many genetic studies. The major drawback of microsatellites is that they need to be isolated de novo from species that are being examined for the first time. The aim of the present paper is to review the various methods of microsatellite isolation described in the literature with the purpose of providing useful guidelines in making appropriate choices among the large number of currently available options. In addition, we propose a fast and easy protocol which is a combination of different published methods.
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            [SSRHunter: development of a local searching software for SSR sites].

            Progress in genome research has made it possible to develop new SSR markers by bioinformatics in a relatively narrow region of genome. To realize it, the first thing is to search for potential SSR sites. Any known methods have more or less defects. Efforts were made to develop a local SSR sites searching software in this study. The resultant software, SSRHunter, could accomplish this task perfectly. Furthermore, SSRHunter could provide automatic pretreatment of sequences, routine arrangement of sequences, sequence transformation and convenient report output.
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              Author and article information

              Journal
              Appl Plant Sci
              Appl Plant Sci
              apps
              Applications in Plant Sciences
              Botanical Society of America
              2168-0450
              July 2016
              13 July 2016
              : 4
              : 7
              : apps.1600004
              Affiliations
              [2 ]Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, People’s Republic of China
              [3 ]Zhuhai College of Jilin University, Zhuhai 519041, People’s Republic of China
              Author notes
              [1]

              This work was supported by the Guangxi Natural Science Foundation of China (2012GXNSFBA053075). The authors are grateful to X. J. Ouyang and L. J. Deng for their help collecting samples in Dinghushan National Nature Reserve. Laboratory work was performed at Kunming Institute of Botany, Chinese Academy of Sciences. The authors are also grateful to Z. R. Zhang for his help in lab work and Prof. Bill Price for assistance with English editing.

              [4 ]Author for correspondence: calljone@ 123456163.com
              Article
              apps1600004
              10.3732/apps.1600004
              4948904
              27437176
              ebc930ce-d821-4612-9ff2-4d5711c03a7a
              © 2016 Zhao et al. Published by the Botanical Society of America

              This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).

              History
              : 7 January 2016
              : 8 April 2016
              Categories
              Primer Note

              begonia fimbristipula,begoniaceae,conservation,microsatellite markers

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